Detailed information of TA system TA089379

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 62399..62663
• Replicon: plasmid pCV839-15-p2
• Accession: NZ_CP024976
• Organism: Escherichia coli strain CV839-15

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: CV83915_RS01045
• Protein ID: WP_001303307.1
• Coordinates: 62511..62663 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	62399..62461 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV83915_RS01030	58501..59571	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
CV83915_RS01035	59590..60798	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	60978..61038	-	61	NuclAT_3	-	-
-	60978..61038	-	61	NuclAT_3	-	-
-	60978..61038	-	61	NuclAT_3	-	-
-	60978..61038	-	61	NuclAT_3	-	-
CV83915_RS01040	61105..62196	-	1092	WP_000426061.1	hypothetical protein	-
-	62399..62461	-	63	NuclAT_2	-	Antitoxin
-	62399..62461	-	63	NuclAT_2	-	Antitoxin
-	62399..62461	-	63	NuclAT_2	-	Antitoxin
-	62399..62461	-	63	NuclAT_2	-	Antitoxin
CV83915_RS01045	62511..62663	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
CV83915_RS01050	62735..62986	-	252	WP_001291965.1	hypothetical protein	-
-	63373..63424	-	52	NuclAT_4	-	-
-	63373..63424	-	52	NuclAT_4	-	-
-	63373..63424	-	52	NuclAT_4	-	-
-	63373..63424	-	52	NuclAT_4	-	-
CV83915_RS29305	63910..64086	-	177	WP_001054900.1	hypothetical protein	-
CV83915_RS01065	64295..64504	-	210	WP_001140545.1	hemolysin expression modulator Hha	-
CV83915_RS01070	64602..65216	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
CV83915_RS01075	65292..67460	-	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2	-	1..195578	195578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T89379 WP_001303307.1 NZ_CP024976:62511-62663 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T89379 NZ_CP024976:62511-62663 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT89379 NZ_CP024976:c62461-62399 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin