Detailed information of TA system TA089350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4859652..4860309
• Replicon: chromosome
• Accession: NZ_CP024956
• Organism: Pectobacterium atrosepticum strain 36A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CVS35_RS22005
• Protein ID: WP_039294444.1
• Coordinates: 4859652..4860023 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CVS35_RS22010
• Protein ID: WP_039294447.1
• Coordinates: 4860010..4860309 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVS35_RS21985	4855908..4857056	-	1149	WP_039294433.1	galactonate dehydratase	-
CVS35_RS21990	4857053..4857670	-	618	WP_039294435.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
CVS35_RS21995	4857654..4858550	-	897	WP_039294438.1	2-dehydro-3-deoxygalactonokinase	-
CVS35_RS22000	4858547..4859245	-	699	WP_039294441.1	D-galactonate utilization transcriptional regulator DgoR	-
CVS35_RS22005	4859652..4860023	+	372	WP_039294444.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CVS35_RS22010	4860010..4860309	+	300	WP_039294447.1	XRE family transcriptional regulator	Antitoxin
CVS35_RS22015	4860347..4862146	-	1800	WP_039294450.1	ABC transporter ATP-binding protein	-
CVS35_RS22020	4862186..4863094	-	909	WP_039294453.1	ABC transporter permease	-
CVS35_RS22025	4863091..4864068	-	978	WP_039294455.1	ABC transporter permease	-
CVS35_RS22030	4864068..4865039	-	972	WP_039294457.1	nucleoside hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14160.08 Da
• Isoelectric Point: 4.8590

>T89350 WP_039294444.1 NZ_CP024956:4859652-4860023 [Pectobacterium atrosepticum]
MAWKIVTRDLFDTWFDEQTEAVQEEVLAHLAILEEDGPQLGRPQVDQIKGSKYKNMKELRVQVGGHPFRTFFAFDRTRKA
IVLCAGDKKGEDEKLFYERMIKIADAEFTSHLISPEVKEDGDA

Nucleotide

• Length: 372 bp

>T89350 NZ_CP024956:4859652-4860023 [Pectobacterium atrosepticum]
TTGGCATGGAAAATAGTAACACGGGATCTATTCGATACTTGGTTTGATGAGCAAACCGAAGCGGTACAGGAAGAGGTTTT
GGCACACCTCGCAATACTCGAAGAGGATGGCCCACAGCTAGGACGACCACAGGTTGATCAGATTAAAGGTTCAAAATACA
AAAATATGAAGGAGCTGCGGGTTCAGGTTGGAGGCCACCCATTCCGAACATTCTTCGCGTTTGACCGGACACGTAAAGCG
ATTGTTTTATGTGCAGGAGATAAGAAAGGCGAGGATGAAAAATTGTTCTACGAACGTATGATTAAGATCGCAGACGCTGA
GTTTACATCGCATCTGATATCACCGGAGGTAAAAGAAGATGGCGACGCTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11019.09 Da
• Isoelectric Point: 10.8728

>AT89350 WP_039294447.1 NZ_CP024956:4860010-4860309 [Pectobacterium atrosepticum]
MATLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELKMSQTQLAAALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPNGKRIALTL

Nucleotide

• Length: 300 bp

>AT89350 NZ_CP024956:4860010-4860309 [Pectobacterium atrosepticum]
ATGGCGACGCTTAAAGAAATGATGGCTAAACGTTCACCGGAGAGCCGTGCCCGTATTGAGGCGCGCACGGCTGAGATCCG
CCAGGAAATCACTTTAGCGAAACTACGCGAAGAACTGAAAATGTCGCAGACGCAGCTTGCAGCAGCATTGGGGGTCAAGC
AGCCATCTATTGTTAAGATGGAAAAGGTTGAAAATGACCCAAAACTGTCAACGCTGAAACGCTATGTAACTGCCCTAGGC
GGTAAGCTTACGCTAGATGTGACCCTACCTAATGGTAAGCGTATCGCGCTTACGTTGTAA