Detailed information of TA system TA089342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 423779..424503
• Replicon: chromosome
• Accession: NZ_CP024956
• Organism: Pectobacterium atrosepticum strain 36A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q6DA89
• Locus tag: CVS35_RS01985
• Protein ID: WP_011091996.1
• Coordinates: 423779..424090 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CVS35_RS01990
• Protein ID: WP_011091997.1
• Coordinates: 424087..424503 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVS35_RS01970	419935..421470	-	1536	WP_011091993.1	carbohydrate porin	-
CVS35_RS01975	421661..422596	-	936	WP_011091994.1	aminoimidazole riboside kinase	-
CVS35_RS01980	423068..423619	+	552	WP_039294652.1	polymer-forming cytoskeletal protein	-
CVS35_RS01985	423779..424090	+	312	WP_011091996.1	type II toxin-antitoxin system HigB family toxin	Toxin
CVS35_RS01990	424087..424503	+	417	WP_011091997.1	helix-turn-helix domain-containing protein	Antitoxin
CVS35_RS01995	424706..425164	+	459	WP_039296801.1	hypothetical protein	-
CVS35_RS02000	425245..426597	-	1353	WP_039296941.1	aspartate aminotransferase family protein	-
CVS35_RS02005	426631..427911	-	1281	WP_039296944.1	MFS transporter	-
CVS35_RS02010	428065..428511	+	447	WP_011092001.1	helix-turn-helix transcriptional regulator	-
CVS35_RS02015	428521..429015	+	495	WP_039296802.1	YhbP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12431.33 Da
• Isoelectric Point: 10.0380

>T89342 WP_011091996.1 NZ_CP024956:423779-424090 [Pectobacterium atrosepticum]
MHVISRAPFDAATNQFPNQAVALADLYRVIKREMYATPEDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKI
FIKHITSHAEYDRLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T89342 NZ_CP024956:423779-424090 [Pectobacterium atrosepticum]
ATGCATGTGATTTCGCGCGCACCTTTTGATGCTGCAACCAACCAGTTTCCGAATCAGGCGGTAGCACTCGCTGACCTATA
TCGGGTTATAAAACGCGAAATGTATGCAACGCCGGAGGATATGAAAAAACGCTTTCCCAGCTTAGATAGGATGAAATATC
GAGAAAAGTGGTGGGTTATTGATATCGGTGGTGGGCATCTTCGAGTGATGTTTTTTGCTGATTTTGAGCGAGGGAAAATC
TTCATCAAGCACATAACATCCCATGCAGAGTACGACAGGCTGACAGAGTATTACCGGAGGAATAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15395.73 Da
• Isoelectric Point: 4.7943

>AT89342 WP_011091997.1 NZ_CP024956:424087-424503 [Pectobacterium atrosepticum]
MMYADAIKAANDLTSIVPFLGGSTSRKDYEDALKLTEYLIESEPDSSLIDMLTAKIDKYENEAPEFAAFNERIKALPAGI
ALLRVLMDQHQLMQSDFEAEIGKKSLVSRILNGERTLTLAHMRALAKRFNIPVSAFVD

Nucleotide

• Length: 417 bp

>AT89342 NZ_CP024956:424087-424503 [Pectobacterium atrosepticum]
ATGATGTATGCAGATGCCATCAAGGCAGCTAACGATCTGACGAGTATCGTACCGTTCCTCGGGGGCAGCACCTCTCGTAA
GGATTATGAGGATGCACTGAAACTCACTGAATATCTGATCGAGAGTGAGCCTGATAGTTCGCTAATCGATATGCTGACTG
CCAAAATTGATAAATACGAAAATGAGGCGCCGGAGTTTGCAGCCTTCAACGAACGTATTAAAGCACTCCCTGCCGGTATC
GCTTTGCTACGTGTCCTCATGGATCAGCACCAGCTAATGCAGAGTGATTTTGAAGCTGAGATCGGTAAAAAGTCTCTGGT
ATCCCGTATTCTAAACGGTGAGCGTACGTTAACACTTGCTCATATGAGAGCTCTCGCTAAGCGATTCAATATTCCCGTGA
GTGCTTTTGTTGATTAA

Structures

Toxin

• AlphaFold DB: Q6DA89