Detailed information of TA system TA089325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 859133..859721
• Replicon: chromosome
• Accession: NZ_CP024948
• Organism: Helicobacter pylori strain B140

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: J0MTE5
• Locus tag: CV728_RS04180
• Protein ID: WP_000921924.1
• Coordinates: 859133..859414 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: -
• Locus tag: CV728_RS04185
• Protein ID: WP_145817277.1
• Coordinates: 859407..859721 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV728_RS04140	854198..854461	+	264	WP_145818277.1	hypothetical protein	-
CV728_RS04145	854458..854757	+	300	WP_001942121.1	hypothetical protein	-
CV728_RS04150	854757..855698	+	942	WP_145817269.1	Flp pilus assembly complex ATPase component TadA	-
CV728_RS04155	855695..856150	+	456	WP_145817271.1	mobilization protein	-
CV728_RS04160	856147..856437	+	291	WP_000159808.1	hypothetical protein	-
CV728_RS04165	856438..856995	+	558	WP_145817273.1	hypothetical protein	-
CV728_RS08545	856996..858724	+	1729	Protein_784	type IV secretory system conjugative DNA transfer family protein	-
CV728_RS04180	859133..859414	-	282	WP_000921924.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
CV728_RS04185	859407..859721	-	315	WP_145817277.1	hypothetical protein	Antitoxin
CV728_RS04190	860016..861929	+	1914	WP_145817279.1	relaxase/mobilization nuclease domain-containing protein	-
CV728_RS04195	861954..863138	-	1185	WP_145817280.1	hypothetical protein	-
CV728_RS04200	863170..863454	-	285	WP_000394655.1	hypothetical protein	-
CV728_RS04205	863533..864189	-	657	WP_001932555.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10901.93 Da
• Isoelectric Point: 10.1597

>T89325 WP_000921924.1 NZ_CP024948:c859414-859133 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRIKESDVASIVDCLKKQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T89325 NZ_CP024948:c859414-859133 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTAAAGAAAGCGA
TGTTGCAAGCATTGTTGATTGCCTAAAAAAACAAAAACCACTCCAACAAAAATATTGTGACCACGCTTTGAGCGGTAATC
TTAAAGGGCTGAGAGAGTGTCATGTCAAGCCGAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12150.60 Da
• Isoelectric Point: 5.2068

>AT89325 WP_145817277.1 NZ_CP024948:c859721-859407 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNAIEKQLLKAEGDKNKAIKKIQECELQEQMIRQVLAQKHSQEKEPTPSLLNTIASKDDPE
YDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT89325 NZ_CP024948:c859721-859407 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACGCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACCAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TACGATGTTTCGTTTGGAGACTTCAATGATTTTTTGCAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Toxin

• AlphaFold DB: A0A518YFA9