Detailed information of TA system TA089319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 109607..110195
• Replicon: chromosome
• Accession: NZ_CP024947
• Organism: Helicobacter pylori strain J182

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: T5CGR9
• Locus tag: CV729_RS00505
• Protein ID: WP_000921933.1
• Coordinates: 109914..110195 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: -
• Locus tag: CV729_RS00500
• Protein ID: WP_145731499.1
• Coordinates: 109607..109921 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV729_RS00475	104803..105054	-	252	WP_001105124.1	hypothetical protein	-
CV729_RS00480	105171..105779	+	609	WP_145731493.1	ParA family protein	-
CV729_RS00485	105861..106145	+	285	WP_000394645.1	hypothetical protein	-
CV729_RS00490	106189..107373	+	1185	WP_145731495.1	hypothetical protein	-
CV729_RS00495	107398..109311	-	1914	WP_145731497.1	relaxase/mobilization nuclease domain-containing protein	-
CV729_RS00500	109607..109921	+	315	WP_145731499.1	hypothetical protein	Antitoxin
CV729_RS00505	109914..110195	+	282	WP_000921933.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
CV729_RS00510	110469..112196	-	1728	WP_194151354.1	type IV secretory system conjugative DNA transfer family protein	-
CV729_RS00515	112197..112754	-	558	WP_145731500.1	hypothetical protein	-
CV729_RS00520	112755..113045	-	291	WP_000159819.1	hypothetical protein	-
CV729_RS00525	113042..113497	-	456	WP_145731502.1	mobilization protein	-
CV729_RS00530	113547..114434	-	888	WP_145731504.1	Flp pilus assembly complex ATPase component TadA	-
CV729_RS00535	114434..114733	-	300	WP_145731506.1	hypothetical protein	-
CV729_RS00540	114730..114993	-	264	WP_145731508.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10875.80 Da
• Isoelectric Point: 9.5726

>T89319 WP_000921933.1 NZ_CP024947:109914-110195 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRITESDVASIVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T89319 NZ_CP024947:109914-110195 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATTTTATCTGGGCGTATTACAGAAAGCGA
TGTTGCAAGCATTGTTGATTGCCTCAAAGAGCAAAAACCACTCCAACAAAAATATTGCGACCATGCTTTGAGCGGCAATC
TTAAAGGGCTGAGAGAGTGTCATGTCAAACCGAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12253.72 Da
• Isoelectric Point: 5.6761

>AT89319 WP_145731499.1 NZ_CP024947:109607-109921 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNSIEKQLLKAEGDKNKAIKKIQECELQERMIRQVLAQKHSQEKEPTQSLLNTIVSKDDPE
YDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT89319 NZ_CP024947:109607-109921 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTATTCTCAATACAATGAAAAGCAACTGCACAATTTCTTAAACTCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACGAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTCTGTTAAATACGATCGTCTCAAAAGATGACCCAGAA
TACGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Toxin

• AlphaFold DB: T5CGR9