Detailed information of TA system TA089296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2944965..2945564
• Replicon: chromosome
• Accession: NZ_CP024937
• Organism: Paraburkholderia hospita strain mHSR1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: CUJ88_RS13155
• Protein ID: WP_201762687.1
• Coordinates: 2945286..2945564 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A244DXM4
• Locus tag: CUJ88_RS13150
• Protein ID: WP_007588944.1
• Coordinates: 2944965..2945273 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUJ88_RS13140	2940079..2941995	+	1917	WP_039901634.1	SMP-30/gluconolactonase/LRE family protein	-
CUJ88_RS13145	2942446..2944404	+	1959	WP_100216065.1	alkaline phosphatase family protein	-
CUJ88_RS50390	2944488..2944652	-	165	WP_158660509.1	hypothetical protein	-
CUJ88_RS13150	2944965..2945273	-	309	WP_007588944.1	HigA family addiction module antidote protein	Antitoxin
CUJ88_RS13155	2945286..2945564	-	279	WP_201762687.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CUJ88_RS13160	2945615..2945794	+	180	Protein_2556	YdaU family protein	-
CUJ88_RS13165	2946151..2946750	-	600	WP_007588949.1	NADPH-dependent F420 reductase	-
CUJ88_RS13170	2946853..2947587	-	735	WP_114221594.1	SDR family oxidoreductase	-
CUJ88_RS13175	2947718..2948293	+	576	WP_035538937.1	TetR/AcrR family transcriptional regulator	-
CUJ88_RS13180	2948698..2950026	-	1329	WP_007588954.1	FAD-binding oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	htpB	2851004..3036978	185974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10529.05 Da
• Isoelectric Point: 9.2613

>T89296 WP_201762687.1 NZ_CP024937:c2945564-2945286 [Paraburkholderia hospita]
MIQTFRCPDTQALFEGRRVPRFANIRTVAERKLTLLHAAMTLDALRSPPGNRLEALKGDRAGQYSIRINDQWRICFGWTA
DGPTNVEIVDYH

Nucleotide

• Length: 279 bp

>T89296 NZ_CP024937:c2945564-2945286 [Paraburkholderia hospita]
ATGATCCAAACGTTCCGATGCCCCGATACACAGGCCCTGTTTGAAGGGCGCCGCGTTCCGAGATTTGCGAACATCCGGAC
TGTTGCCGAACGTAAGCTGACACTGCTCCACGCGGCCATGACGCTCGACGCGTTGAGGTCGCCGCCGGGCAACCGGCTCG
AAGCGCTTAAGGGCGACCGCGCCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTTGGATGGACGGCC
GACGGCCCGACGAACGTTGAAATCGTCGACTACCACTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11611.47 Da
• Isoelectric Point: 8.5682

>AT89296 WP_007588944.1 NZ_CP024937:c2945273-2944965 [Paraburkholderia hospita]
MVKNGMRPVHPGEILREEYLVPLGMSANALATALHVTAGRINDIVRERRGITPDTALRLARYFGGDAQTWMNLQLMYDMK
IAERDIARKIEREIEPRELAHA

Nucleotide

• Length: 309 bp

>AT89296 NZ_CP024937:c2945273-2944965 [Paraburkholderia hospita]
ATGGTCAAGAACGGTATGCGCCCGGTCCACCCAGGCGAGATCCTCCGCGAGGAATACCTCGTGCCGCTCGGCATGAGCGC
GAATGCGCTGGCGACGGCGTTGCACGTTACCGCCGGACGGATCAACGACATCGTGCGCGAGCGCCGCGGCATCACGCCGG
ACACTGCATTGCGCCTGGCGCGATATTTCGGCGGCGACGCGCAGACGTGGATGAACCTGCAACTCATGTACGACATGAAG
ATTGCCGAGCGCGACATTGCACGGAAAATCGAGCGCGAAATCGAGCCGCGCGAACTGGCGCACGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A244DXM4