Detailed information of TA system TA089277

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51415..51679
• Replicon: plasmid pKPNH54.2
• Accession: NZ_CP024918
• Organism: Klebsiella pneumoniae strain NH54

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: CU643_RS28335
• Protein ID: WP_024179505.1
• Coordinates: 51527..51679 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	51415..51475 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU643_RS28320	46654..48945	-	2292	WP_077265944.1	conjugal transfer protein TrbC	-
CU643_RS28325	48938..50008	-	1071	WP_064148470.1	IncI1-type conjugal transfer protein TrbB	-
CU643_RS28330	50027..51235	-	1209	WP_064148469.1	IncI1-type conjugal transfer protein TrbA	-
-	51415..51475	-	61	NuclAT_0	-	Antitoxin
-	51415..51475	-	61	NuclAT_0	-	Antitoxin
-	51415..51475	-	61	NuclAT_0	-	Antitoxin
-	51415..51475	-	61	NuclAT_0	-	Antitoxin
CU643_RS28335	51527..51679	+	153	WP_024179505.1	Hok/Gef family protein	Toxin
CU643_RS28340	51751..52002	-	252	WP_001291962.1	hypothetical protein	-
-	52395..52446	-	52	NuclAT_1	-	-
-	52395..52446	-	52	NuclAT_1	-	-
-	52395..52446	-	52	NuclAT_1	-	-
-	52395..52446	-	52	NuclAT_1	-	-
CU643_RS29475	53335..53511	-	177	WP_180335155.1	hypothetical protein	-
CU643_RS28350	53904..54113	+	210	WP_094308840.1	HEAT repeat domain-containing protein	-
CU643_RS28355	54185..54835	-	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..81261	81261

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5743.07 Da
• Isoelectric Point: 8.7948

>T89277 WP_024179505.1 NZ_CP024918:51527-51679 [Klebsiella pneumoniae]
MPQRTFLMMLIVVCVTILCFVWIVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T89277 NZ_CP024918:51527-51679 [Klebsiella pneumoniae]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATTGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT89277 NZ_CP024918:c51475-51415 [Klebsiella pneumoniae]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin