Detailed information of TA system TA089238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3000207..3000960
• Replicon: chromosome
• Accession: NZ_CP024905
• Organism: Paraburkholderia caledonica strain PHRS4

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2Z5MEV4
• Locus tag: CUJ87_RS13180
• Protein ID: WP_027776364.1
• Coordinates: 3000207..3000488 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CUJ87_RS13185
• Protein ID: WP_027776363.1
• Coordinates: 3000505..3000960 (+)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUJ87_RS13165	2995680..2996252	+	573	WP_027776367.1	TMEM165/GDT1 family protein	-
CUJ87_RS13170	2996332..2997042	-	711	WP_027776366.1	DUF4136 domain-containing protein	-
CUJ87_RS13175	2997300..3000005	+	2706	WP_114197477.1	aminopeptidase N	-
CUJ87_RS13180	3000207..3000488	+	282	WP_027776364.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CUJ87_RS13185	3000505..3000960	+	456	WP_027776363.1	HigA family addiction module antidote protein	Antitoxin
CUJ87_RS13190	3001092..3002108	+	1017	WP_020069041.1	class 1 fructose-bisphosphatase	-
CUJ87_RS33065	3003206..3003355	+	150	WP_006050185.1	hypothetical protein	-
CUJ87_RS13205	3003635..3004231	+	597	WP_027776361.1	chorismate mutase	-
CUJ87_RS13210	3004338..3005489	-	1152	WP_027776360.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-84)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10675.32 Da
• Isoelectric Point: 8.5477

>T89238 WP_027776364.1 NZ_CP024905:3000207-3000488 [Paraburkholderia caledonica]
MITTFGDKATARIFQGKFVHTLPRHIQALAYRKLLMIDAADCIQSLHTPPGNRLEALQGQRSGQWSIRINAQWRICFHFI
EGEALNVEIVDYH

Nucleotide

• Length: 282 bp

>T89238 NZ_CP024905:3000207-3000488 [Paraburkholderia caledonica]
ATGATCACGACATTTGGCGACAAGGCTACGGCGAGAATCTTTCAGGGCAAGTTCGTGCATACGCTGCCGCGTCATATCCA
GGCGCTCGCGTACCGCAAGTTACTGATGATCGACGCTGCAGACTGCATCCAAAGCTTGCACACGCCGCCCGGCAACCGGC
TCGAAGCACTGCAAGGCCAGCGCAGCGGGCAATGGAGCATTCGGATCAATGCGCAGTGGCGAATCTGTTTCCACTTTATC
GAAGGCGAGGCGCTGAATGTTGAGATTGTCGACTATCACTGA

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 16278.60 Da
• Isoelectric Point: 6.4791

>AT89238 WP_027776363.1 NZ_CP024905:3000505-3000960 [Paraburkholderia caledonica]
MVIKRSDLDSIDFSDIDAGDSIEEIHPGEILRSEFLEPLGMSVNALALALRVPAPRINDVVRGKRAISADTALRLERYFG
ASAQFWLNLQIAYDLRVATAAAGEQIEREIEPMPKADRPKLPKVSAEHAQAAALAACVSGNATGGKARRKG

Nucleotide

• Length: 456 bp

>AT89238 NZ_CP024905:3000505-3000960 [Paraburkholderia caledonica]
ATGGTCATCAAACGCTCCGATCTGGACAGTATCGATTTCTCCGACATCGACGCGGGAGACAGCATCGAGGAGATCCATCC
GGGTGAGATTCTGCGCAGCGAGTTTCTCGAACCGCTCGGCATGTCAGTCAACGCGCTTGCGCTCGCGTTGCGCGTACCGG
CGCCGCGCATCAACGACGTGGTCCGCGGCAAGCGCGCAATTTCCGCCGACACCGCGCTGCGCCTCGAGCGCTATTTCGGC
GCGAGCGCGCAGTTCTGGCTCAACCTGCAGATCGCATACGACCTGCGCGTTGCCACGGCGGCAGCGGGCGAGCAGATCGA
ACGCGAGATAGAACCCATGCCTAAGGCGGACCGCCCGAAGCTGCCGAAGGTATCGGCGGAACACGCCCAGGCAGCGGCGC
TCGCAGCATGCGTGAGCGGCAACGCGACGGGCGGAAAGGCCCGGCGCAAAGGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z5MEV4