Detailed information of TA system TA089220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4668054..4668721
• Replicon: chromosome
• Accession: NZ_CP024901
• Organism: Photorhabdus laumondii subsp. laumondii strain TT01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q7N0B2
• Locus tag: PluTT01m_RS20490
• Protein ID: WP_011148113.1
• Coordinates: 4668054..4668377 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PluTT01m_RS20495
• Protein ID: WP_011148114.1
• Coordinates: 4668425..4668721 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PluTT01m_RS20470	4664431..4666275	+	1845	WP_011148111.1	RNA polymerase sigma factor RpoD	-
PluTT01m_RS20485	4667098..4667670	-	573	WP_011148112.1	hypothetical protein	-
PluTT01m_RS20490	4668054..4668377	+	324	WP_011148113.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PluTT01m_RS20495	4668425..4668721	+	297	WP_011148114.1	helix-turn-helix domain-containing protein	Antitoxin
PluTT01m_RS26295	4669147..4669347	+	201	WP_125026254.1	hypothetical protein	-
PluTT01m_RS20500	4669735..4670670	-	936	Protein_3916	hypothetical protein	-
PluTT01m_RS20505	4670785..4671729	-	945	WP_011148118.1	class I SAM-dependent methyltransferase	-
PluTT01m_RS20510	4671867..4672400	-	534	WP_011148119.1	isopentenyl-diphosphate Delta-isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (34-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-92)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12550.65 Da
• Isoelectric Point: 10.1702

>T89220 WP_011148113.1 NZ_CP024901:4668054-4668377 [Photorhabdus laumondii subsp. laumondii]
MEYLVFIETPVFSRERAELLTDDEFRLLQEHLLKNHEQGSTISATGGCKKIRWQRERIGKRGGVRIIYYAITKTGRLYLL
LIYPKNKKDDLTPKEKEVLKAISNKLQ

Nucleotide

• Length: 324 bp

>T89220 NZ_CP024901:4668054-4668377 [Photorhabdus laumondii subsp. laumondii]
ATGGAATATCTGGTTTTTATTGAAACTCCCGTATTTAGTAGAGAACGAGCAGAACTGTTAACCGATGATGAATTTAGGCT
ATTACAGGAGCACTTACTAAAGAATCACGAGCAGGGTAGTACCATCAGCGCTACTGGAGGATGTAAAAAGATTCGTTGGC
AGCGAGAAAGGATAGGAAAACGTGGCGGAGTAAGAATTATCTACTATGCCATAACCAAAACTGGGAGGCTCTATCTCCTT
TTGATTTACCCTAAAAATAAAAAAGATGACTTAACTCCAAAAGAAAAAGAGGTACTTAAAGCCATCAGTAACAAATTACA
GTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11269.92 Da
• Isoelectric Point: 6.2274

>AT89220 WP_011148114.1 NZ_CP024901:4668425-4668721 [Photorhabdus laumondii subsp. laumondii]
MDKRLFNDLVESMNQMVAIEKGELSVFDENIHYHRLPDVKNLRETFGLKQSEFAEAVGVSTSLIQSWEQHRRIPSGSSLK
LLKMLERNPALINELSTL

Nucleotide

• Length: 297 bp

>AT89220 NZ_CP024901:4668425-4668721 [Photorhabdus laumondii subsp. laumondii]
ATGGACAAAAGACTTTTTAACGATTTGGTTGAAAGCATGAATCAGATGGTAGCAATTGAAAAAGGCGAACTTTCTGTTTT
TGATGAAAATATCCATTACCATCGCCTGCCTGATGTAAAAAACTTACGTGAAACCTTTGGCTTAAAACAAAGTGAATTTG
CCGAAGCTGTTGGCGTTTCTACATCTCTAATTCAAAGCTGGGAACAGCACCGCCGCATTCCTTCTGGTTCATCACTGAAA
CTACTTAAGATGCTTGAACGTAATCCTGCGCTTATTAATGAATTAAGCACACTTTAA

Structures

Toxin

• AlphaFold DB: A0A6L9JJS0