Detailed information of TA system TA089198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 993018..993588
• Replicon: chromosome
• Accession: NZ_CP024901
• Organism: Photorhabdus laumondii subsp. laumondii strain TT01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q7N875
• Locus tag: PluTT01m_RS04465
• Protein ID: WP_011145234.1
• Coordinates: 993307..993588 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PluTT01m_RS04460
• Protein ID: WP_011145233.1
• Coordinates: 993018..993296 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PluTT01m_RS04430	989171..989434	+	264	WP_011145227.1	helix-turn-helix domain-containing protein	-
PluTT01m_RS04435	989804..990349	+	546	WP_011145228.1	hypoxanthine phosphoribosyltransferase	-
PluTT01m_RS04440	990570..990848	+	279	WP_011145229.1	DUF1778 domain-containing protein	-
PluTT01m_RS04445	990853..991353	+	501	WP_011145230.1	GNAT family N-acetyltransferase	-
PluTT01m_RS04450	991508..992047	+	540	WP_041379935.1	SocA family protein	-
PluTT01m_RS04455	992382..992918	+	537	WP_011145232.1	hypoxanthine phosphoribosyltransferase	-
PluTT01m_RS04460	993018..993296	-	279	WP_011145233.1	HigA family addiction module antidote protein	Antitoxin
PluTT01m_RS04465	993307..993588	-	282	WP_011145234.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PluTT01m_RS04475	995108..995761	-	654	WP_011145235.1	carbonate dehydratase	-
PluTT01m_RS04480	995949..996860	+	912	WP_011145236.1	ABC transporter ATP-binding protein	-
PluTT01m_RS04485	996872..997642	+	771	WP_011145237.1	ABC transporter permease	-
PluTT01m_RS04490	997721..998101	-	381	WP_011145238.1	aspartate 1-decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-63)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10633.28 Da
• Isoelectric Point: 10.4815

>T89198 WP_011145234.1 NZ_CP024901:c993588-993307 [Photorhabdus laumondii subsp. laumondii]
MIKSFKHKGLEKFFKTGSTAGIQPKHALKLQIQLTALNTAKKPEDMNAPSWKLHPLKGANLSGHWAISVNSNWRLTFRFE
GEDIILVNYQDYH

Nucleotide

• Length: 282 bp

>T89198 NZ_CP024901:c993588-993307 [Photorhabdus laumondii subsp. laumondii]
ATGATTAAAAGCTTCAAACACAAAGGATTGGAAAAATTTTTCAAAACTGGTTCTACCGCTGGCATTCAGCCTAAGCATGC
CCTAAAGCTGCAAATCCAGCTAACTGCATTGAATACGGCTAAAAAACCAGAAGATATGAATGCCCCTAGCTGGAAACTTC
ACCCACTAAAAGGAGCAAATCTTAGCGGTCACTGGGCAATATCTGTTAACAGTAATTGGCGTTTAACATTTAGATTTGAG
GGCGAAGATATAATTCTCGTTAATTATCAAGATTATCACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9992.63 Da
• Isoelectric Point: 9.7351

>AT89198 WP_011145233.1 NZ_CP024901:c993296-993018 [Photorhabdus laumondii subsp. laumondii]
MKMHKPAHPGIVLREYLGDISVTEAAKALGITRAALSRILNGNAGISADMALRLEAALGTSAEMWTGMQSQYELWIASQH
KRPEIKPILAHS

Nucleotide

• Length: 279 bp

>AT89198 NZ_CP024901:c993296-993018 [Photorhabdus laumondii subsp. laumondii]
ATGAAAATGCATAAACCTGCACACCCAGGCATAGTTTTGCGTGAATATCTTGGTGACATATCTGTCACGGAAGCAGCAAA
GGCTCTCGGTATAACCAGAGCTGCACTATCCCGCATATTAAACGGCAATGCAGGAATATCTGCTGATATGGCCCTGCGAC
TGGAAGCCGCATTAGGTACTAGTGCCGAAATGTGGACAGGAATGCAGTCCCAATATGAACTGTGGATTGCCTCACAGCAC
AAAAGACCAGAAATAAAACCAATACTGGCTCACTCCTGA

Structures

Toxin

• AlphaFold DB: Q7N875