Detailed information of TA system TA089186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4489747..4490414
• Replicon: chromosome
• Accession: NZ_CP024900
• Organism: Photorhabdus laumondii subsp. laumondii strain DJC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PluDJC_RS19810
• Protein ID: WP_109791828.1
• Coordinates: 4489747..4490070 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PluDJC_RS19815
• Protein ID: WP_011148114.1
• Coordinates: 4490118..4490414 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PluDJC_RS19790	4486124..4487968	+	1845	WP_109791827.1	RNA polymerase sigma factor RpoD	-
PluDJC_RS19805	4488791..4489363	-	573	WP_082302905.1	Bro-N domain-containing protein	-
PluDJC_RS19810	4489747..4490070	+	324	WP_109791828.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PluDJC_RS19815	4490118..4490414	+	297	WP_011148114.1	helix-turn-helix domain-containing protein	Antitoxin
PluDJC_RS25760	4490840..4491040	+	201	WP_125026254.1	hypothetical protein	-
PluDJC_RS19820	4491428..4492354	-	927	WP_112872161.1	hypothetical protein	-
PluDJC_RS19825	4492475..4493419	-	945	WP_109791830.1	class I SAM-dependent methyltransferase	-
PluDJC_RS19830	4493557..4494091	-	535	Protein_3803	isopentenyl-diphosphate Delta-isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (34-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-92)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12522.63 Da
• Isoelectric Point: 10.1456

>T89186 WP_109791828.1 NZ_CP024900:4489747-4490070 [Photorhabdus laumondii subsp. laumondii]
MEYLVFIETPVFSKERAELLTDDEFRLLQEHLLKNHEQGSTISATGGCKKIRWQRERIGKRGGVRIIYYAITKTGRLYLL
LIYPKNKKDDLTPKEKEVLKAISNKLQ

Nucleotide

• Length: 324 bp

>T89186 NZ_CP024900:4489747-4490070 [Photorhabdus laumondii subsp. laumondii]
ATGGAATATCTGGTTTTTATTGAAACTCCCGTATTTAGTAAAGAACGAGCAGAACTGTTAACCGATGATGAATTTAGGCT
ATTACAGGAGCACTTACTAAAGAATCACGAGCAGGGTAGTACCATCAGCGCTACTGGAGGATGTAAAAAGATTCGTTGGC
AGCGAGAAAGGATAGGAAAACGTGGCGGAGTAAGAATTATCTACTATGCCATAACCAAAACTGGGAGGCTCTATCTCCTT
TTGATTTACCCTAAAAATAAAAAAGATGACTTAACTCCAAAAGAAAAAGAGGTACTTAAAGCCATCAGTAACAAATTACA
GTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11269.92 Da
• Isoelectric Point: 6.2274

>AT89186 WP_011148114.1 NZ_CP024900:4490118-4490414 [Photorhabdus laumondii subsp. laumondii]
MDKRLFNDLVESMNQMVAIEKGELSVFDENIHYHRLPDVKNLRETFGLKQSEFAEAVGVSTSLIQSWEQHRRIPSGSSLK
LLKMLERNPALINELSTL

Nucleotide

• Length: 297 bp

>AT89186 NZ_CP024900:4490118-4490414 [Photorhabdus laumondii subsp. laumondii]
ATGGACAAAAGACTTTTTAACGATCTGGTTGAAAGCATGAATCAGATGGTAGCAATTGAAAAAGGCGAACTTTCTGTTTT
TGATGAAAATATCCATTACCATCGCCTGCCTGATGTAAAAAACTTACGTGAAACCTTTGGCTTAAAACAAAGTGAATTTG
CCGAAGCTGTTGGCGTTTCTACATCTCTAATTCAAAGCTGGGAACAGCACCGCCGCATTCCTTCTGGTTCATCACTGAAA
CTACTTAAGATGCTTGAACGTAATCCTGCGCTTATTAATGAATTAAGCACACTTTAA