Detailed information of TA system TA089178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3334477..3335104
• Replicon: chromosome
• Accession: NZ_CP024900
• Organism: Photorhabdus laumondii subsp. laumondii strain DJC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q7N374
• Locus tag: PluDJC_RS14950
• Protein ID: WP_011147065.1
• Coordinates: 3334477..3334806 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PluDJC_RS14955
• Protein ID: WP_041380121.1
• Coordinates: 3334796..3335104 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PluDJC_RS14935	3331192..3331824	+	633	WP_011147062.1	SDR family oxidoreductase	-
PluDJC_RS14940	3331869..3332186	-	318	WP_011147063.1	DUF496 family protein	-
PluDJC_RS14945	3333220..3333450	+	231	WP_041380119.1	hypothetical protein	-
PluDJC_RS14950	3334477..3334806	+	330	WP_011147065.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PluDJC_RS14955	3334796..3335104	+	309	WP_041380121.1	helix-turn-helix domain-containing protein	Antitoxin
PluDJC_RS14960	3335260..3336690	+	1431	WP_011147067.1	exodeoxyribonuclease I	-
PluDJC_RS14965	3336818..3338533	-	1716	WP_011147068.1	D-lactate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12899.94 Da
• Isoelectric Point: 9.7776

>T89178 WP_011147065.1 NZ_CP024900:3334477-3334806 [Photorhabdus laumondii subsp. laumondii]
MKYTIYYYSTEVENEILALPDTLQARYIRYSERMREHGANLGEPHTEAFGNGLFELRLKGAEGIARVFYCTMIGHRIVML
HSFVKKTQKTPLKERRKAEVRMKEVKNDW

Nucleotide

• Length: 330 bp

>T89178 NZ_CP024900:3334477-3334806 [Photorhabdus laumondii subsp. laumondii]
ATGAAATATACTATCTATTACTATAGCACCGAGGTGGAGAACGAGATCCTTGCTCTCCCTGACACACTTCAAGCCCGTTA
CATCAGGTATTCCGAAAGAATGCGAGAACATGGTGCCAACCTCGGAGAACCACACACAGAAGCGTTTGGTAATGGGTTGT
TTGAACTCAGACTCAAAGGGGCTGAAGGAATTGCTCGCGTATTCTATTGCACAATGATTGGGCACCGTATAGTGATGCTG
CACAGCTTCGTGAAGAAAACACAAAAGACCCCACTTAAAGAACGCAGGAAAGCCGAAGTCAGAATGAAAGAGGTAAAAAA
TGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11170.03 Da
• Isoelectric Point: 10.2817

>AT89178 WP_041380121.1 NZ_CP024900:3334796-3335104 [Photorhabdus laumondii subsp. laumondii]
MTGKYTPPTMTHDEMVAKMLSNPAVKAEYEKLEQEFALLDELLAARKKAGLTQAQVAERMGTKATAITRLESRLASGTQG
PSYNTLKKYAAAVGKKLQIRLV

Nucleotide

• Length: 309 bp

>AT89178 NZ_CP024900:3334796-3335104 [Photorhabdus laumondii subsp. laumondii]
ATGACTGGTAAATATACCCCTCCAACCATGACACACGATGAAATGGTTGCAAAAATGTTGTCAAATCCGGCTGTAAAAGC
TGAATACGAGAAACTGGAACAAGAATTTGCATTGCTTGATGAACTGCTAGCTGCACGCAAAAAGGCCGGGCTAACACAAG
CGCAAGTAGCTGAACGCATGGGAACCAAAGCAACAGCCATCACACGGCTTGAAAGCCGGCTAGCTTCTGGCACACAAGGG
CCTTCGTATAATACATTGAAGAAATATGCAGCAGCTGTCGGTAAAAAATTACAGATACGCCTAGTATGA

Structures

Toxin

• AlphaFold DB: A0A6L9JI68