Detailed information of TA system TA089177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2939082..2939686
• Replicon: chromosome
• Accession: NZ_CP024900
• Organism: Photorhabdus laumondii subsp. laumondii strain DJC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q7N418
• Locus tag: PluDJC_RS13355
• Protein ID: WP_011146760.1
• Coordinates: 2939375..2939686 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PluDJC_RS13350
• Protein ID: WP_109791601.1
• Coordinates: 2939082..2939372 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PluDJC_RS13330	2934938..2935387	-	450	WP_011146754.1	phage tail protein	-
PluDJC_RS13335	2936198..2936791	+	594	WP_113046567.1	winged helix-turn-helix domain-containing protein	-
PluDJC_RS13340	2937320..2937664	+	345	WP_011146757.1	hypothetical protein	-
PluDJC_RS13345	2938126..2938719	+	594	WP_011146758.1	winged helix-turn-helix domain-containing protein	-
PluDJC_RS13350	2939082..2939372	-	291	WP_109791601.1	helix-turn-helix domain-containing protein	Antitoxin
PluDJC_RS13355	2939375..2939686	-	312	WP_011146760.1	hypothetical protein	Toxin
PluDJC_RS13360	2940140..2940910	-	771	WP_011146762.1	DUF2829 domain-containing protein	-
PluDJC_RS13365	2941279..2942117	-	839	Protein_2560	IS5 family transposase	-
PluDJC_RS13370	2942167..2942424	-	258	WP_011146765.1	type II toxin-antitoxin system YafO family toxin	-
PluDJC_RS13375	2942533..2943105	-	573	WP_125026236.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-87)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11976.74 Da
• Isoelectric Point: 8.0254

>T89177 WP_011146760.1 NZ_CP024900:c2939686-2939375 [Photorhabdus laumondii subsp. laumondii]
MIFIETHIFTEDCKELLSDDEYREFQQYLADNPIAGDVIQHTGGLRKVRWASGSKGKRGGVRIIYYYKVDVSHIRLLLIY
KKGIKDDLSESEKKVLRALNEGW

Nucleotide

• Length: 312 bp

>T89177 NZ_CP024900:c2939686-2939375 [Photorhabdus laumondii subsp. laumondii]
ATGATATTTATTGAGACCCATATCTTCACTGAAGATTGTAAAGAGTTGTTAAGTGACGATGAATATCGTGAATTTCAGCA
ATATCTCGCAGACAATCCTATTGCTGGTGATGTTATCCAACACACTGGTGGCTTACGTAAAGTCAGATGGGCATCTGGGA
GTAAAGGTAAGCGGGGTGGTGTGCGCATTATTTATTACTATAAAGTAGATGTCTCACACATCAGATTGTTACTGATTTAC
AAAAAAGGCATCAAGGATGACCTTAGTGAATCAGAAAAGAAAGTCTTGAGAGCACTAAATGAGGGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10664.37 Da
• Isoelectric Point: 10.9696

>AT89177 WP_109791601.1 NZ_CP024900:c2939372-2939082 [Photorhabdus laumondii subsp. laumondii]
MDSKLFSRLTESMTQMNEIINGERAASRETRVEAVKVKNIRQATGLTQTGFAKLISVNVGTLRNWEQGRREPTGPAKALL
KAIEKDPVHVLKALSM

Nucleotide

• Length: 291 bp

>AT89177 NZ_CP024900:c2939372-2939082 [Photorhabdus laumondii subsp. laumondii]
ATGGATAGTAAATTATTTTCCAGGCTAACTGAAAGCATGACACAGATGAACGAAATTATTAATGGCGAACGTGCTGCCTC
TCGTGAAACTCGCGTTGAAGCAGTAAAAGTAAAGAATATTCGCCAGGCAACCGGTTTAACTCAGACCGGTTTTGCTAAGT
TGATTTCCGTTAATGTTGGAACTCTGCGCAACTGGGAACAGGGAAGGCGTGAGCCAACCGGACCAGCAAAAGCCCTGTTG
AAGGCAATAGAGAAAGATCCGGTTCATGTTTTAAAGGCGTTATCTATGTAA

Structures

Toxin

• AlphaFold DB: A0A6L9JR19