Detailed information of TA system TA089149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3263549..3264151
• Replicon: chromosome
• Accession: NZ_CP024889
• Organism: Escherichia coli strain AR_0019

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AM346_RS17165
• Protein ID: WP_000897305.1
• Coordinates: 3263840..3264151 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM346_RS17160
• Protein ID: WP_000356397.1
• Coordinates: 3263549..3263839 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM346_RS17130	3259475..3260377	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AM346_RS17135	3260374..3261009	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AM346_RS17140	3261006..3261935	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AM346_RS17145	3262265..3262507	-	243	WP_001086388.1	hypothetical protein	-
AM346_RS17150	3262726..3262944	-	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
AM346_RS17160	3263549..3263839	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AM346_RS17165	3263840..3264151	-	312	WP_000897305.1	hypothetical protein	Toxin
AM346_RS17170	3264380..3265288	+	909	WP_001162704.1	alpha/beta hydrolase	-
AM346_RS17175	3265352..3266293	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AM346_RS17180	3266338..3266775	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AM346_RS17185	3266772..3267644	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AM346_RS17190	3267638..3268237	-	600	WP_001295269.1	glucose-1-phosphatase	-
AM346_RS17195	3268336..3269121	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T89149 WP_000897305.1 NZ_CP024889:c3264151-3263840 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T89149 NZ_CP024889:c3264151-3263840 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT89149 WP_000356397.1 NZ_CP024889:c3263839-3263549 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT89149 NZ_CP024889:c3263839-3263549 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7