Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2973358..2973899 Replicon chromosome
Accession NZ_CP024889
Organism Escherichia coli strain AR_0019

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag AM346_RS15730 Protein ID WP_000615976.1
Coordinates 2973621..2973899 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AM346_RS15725 Protein ID WP_000729704.1
Coordinates 2973358..2973618 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM346_RS15700 2969141..2969926 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
AM346_RS15705 2969898..2971610 + 1713 Protein_2773 flagellar biosynthesis protein FlhA -
AM346_RS15710 2971715..2971993 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AM346_RS15715 2971986..2972342 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
AM346_RS15720 2972399..2973172 - 774 WP_000543895.1 C40 family peptidase -
AM346_RS15725 2973358..2973618 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AM346_RS15730 2973621..2973899 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AM346_RS15735 2974055..2974795 + 741 WP_001225679.1 murein L,D-transpeptidase -
AM346_RS15740 2974766..2975533 - 768 WP_000333380.1 class II glutamine amidotransferase -
AM346_RS15745 2975739..2976317 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2971986..2983511 11525


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T89148 WP_000615976.1 NZ_CP024889:2973621-2973899 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T89148 NZ_CP024889:2973621-2973899 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT89148 WP_000729704.1 NZ_CP024889:2973358-2973618 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT89148 NZ_CP024889:2973358-2973618 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References