Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1213946..1214448 Replicon chromosome
Accession NZ_CP024889
Organism Escherichia coli strain AR_0019

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM346_RS06615 Protein ID WP_000767829.1
Coordinates 1214194..1214448 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM346_RS06610 Protein ID WP_001259255.1
Coordinates 1213946..1214197 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM346_RS06580 1209124..1210191 - 1068 WP_000080130.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AM346_RS06585 1210191..1211261 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
AM346_RS06590 1211258..1212562 - 1305 WP_001542955.1 histidinol dehydrogenase -
AM346_RS06595 1212568..1213467 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
AM346_RS06605 1213613..1213663 - 51 WP_001364200.1 his operon leader peptide -
AM346_RS06610 1213946..1214197 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM346_RS06615 1214194..1214448 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM346_RS06620 1214531..1215355 + 825 WP_000754758.1 SDR family oxidoreductase -
AM346_RS06625 1215401..1216330 + 930 WP_000803351.1 LysR family transcriptional regulator -
AM346_RS06630 1216545..1216607 + 63 WP_010723108.1 membrane protein YoeI -
AM346_RS06635 1216597..1217955 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM346_RS06640 1218134..1219192 + 1059 WP_000492346.1 transport protein YeeE -
AM346_RS06645 1219206..1219433 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T89135 WP_000767829.1 NZ_CP024889:1214194-1214448 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T89135 NZ_CP024889:1214194-1214448 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT89135 WP_001259255.1 NZ_CP024889:1213946-1214197 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT89135 NZ_CP024889:1213946-1214197 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References