Detailed information of TA system TA089127

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 65430..65700
• Replicon: plasmid tig00000138_pilon
• Accession: NZ_CP024888
• Organism: Escherichia coli strain AR_0019

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM346_RS00430
• Protein ID: WP_001312861.1
• Coordinates: 65542..65700 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	65430..65493 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM346_RS00405	61200..61727	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
AM346_RS00410	61785..62018	+	234	WP_000006003.1	DUF905 family protein	-
AM346_RS00415	62079..64043	+	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
AM346_RS00420	64112..64546	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
AM346_RS00425	64543..65262	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	65274..65498	+	225	NuclAT_0	-	-
-	65274..65498	+	225	NuclAT_0	-	-
-	65274..65498	+	225	NuclAT_0	-	-
-	65274..65498	+	225	NuclAT_0	-	-
-	65430..65493	-	64	-	-	Antitoxin
AM346_RS00430	65542..65700	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM346_RS00450	66616..66912	+	297	WP_001272251.1	hypothetical protein	-
AM346_RS00455	67023..67844	+	822	WP_001234445.1	DUF945 domain-containing protein	-
AM346_RS00460	68141..68743	-	603	Protein_77	transglycosylase SLT domain-containing protein	-
AM346_RS00465	69064..69447	+	384	WP_001151538.1	relaxosome protein TraM	-
AM346_RS00470	69634..70323	+	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..76754	76754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T89127 WP_001312861.1 NZ_CP024888:65542-65700 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T89127 NZ_CP024888:65542-65700 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT89127 NZ_CP024888:c65493-65430 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin