Detailed information of TA system TA089123

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31597..31850
• Replicon: plasmid tig00000138_pilon
• Accession: NZ_CP024888
• Organism: Escherichia coli strain AR_0019

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: AM346_RS00175
• Protein ID: WP_001336447.1
• Coordinates: 31701..31850 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	31597..31653 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM346_RS00140	27509..28255	+	747	WP_019842127.1	conjugal transfer pilus acetylase TraX	-
AM346_RS00145	28310..28867	+	558	WP_000139329.1	fertility inhibition protein FinO	-
AM346_RS00155	29964..30425	+	462	WP_019842128.1	thermonuclease family protein	-
AM346_RS24475	30528..30912	+	385	Protein_30	hypothetical protein	-
AM346_RS00165	31065..31502	+	438	WP_000872609.1	hypothetical protein	-
-	31597..31653	-	57	NuclAT_1	-	Antitoxin
-	31597..31653	-	57	NuclAT_1	-	Antitoxin
-	31597..31653	-	57	NuclAT_1	-	Antitoxin
-	31597..31653	-	57	NuclAT_1	-	Antitoxin
AM346_RS00175	31701..31850	+	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM346_RS00180	32135..32392	+	258	WP_000084404.1	replication regulatory protein RepA	-
AM346_RS00190	32628..32702	+	75	WP_001365705.1	RepA leader peptide Tap	-
AM346_RS00195	32695..33552	+	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
AM346_RS00210	34255..34515	+	261	WP_019842130.1	hypothetical protein	-
AM346_RS00215	34622..34885	+	264	WP_001089473.1	hypothetical protein	-
AM346_RS00220	34875..35174	+	300	WP_001528606.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AM346_RS00225	35210..35875	-	666	WP_001535733.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..76754	76754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T89123 WP_001336447.1 NZ_CP024888:31701-31850 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T89123 NZ_CP024888:31701-31850 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT89123 NZ_CP024888:c31653-31597 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin