Detailed information of TA system TA089120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4621161..4621855
• Replicon: chromosome
• Accession: NZ_CP024886
• Organism: Escherichia coli strain AR_0017

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: AM344_RS24125
• Protein ID: WP_001263500.1
• Coordinates: 4621457..4621855 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AM344_RS24120
• Protein ID: WP_000554758.1
• Coordinates: 4621161..4621454 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM344_RS24100	4616599..4617096	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
AM344_RS27290	4617091..4617486	-	396	WP_113993547.1	hypothetical protein	-
AM344_RS24105	4617514..4619226	-	1713	Protein_4417	flagellar biosynthesis protein FlhA	-
AM344_RS24110	4619198..4619983	+	786	WP_061301231.1	putative lateral flagellar export/assembly protein LafU	-
AM344_RS24115	4620054..4621109	+	1056	WP_001226168.1	DNA polymerase IV	-
AM344_RS24120	4621161..4621454	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AM344_RS24125	4621457..4621855	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AM344_RS24130	4621865..4622317	+	453	WP_071778924.1	GNAT family N-acetyltransferase	-
AM344_RS24135	4622507..4623646	+	1140	WP_000521561.1	RNA ligase RtcB family protein	-
AM344_RS24140	4623643..4624257	+	615	WP_000602123.1	peptide chain release factor H	-
AM344_RS24145	4624314..4625771	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
AM344_RS24150	4626032..4626490	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	4600276..4622317	22041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T89120 WP_001263500.1 NZ_CP024886:4621457-4621855 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T89120 NZ_CP024886:4621457-4621855 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT89120 WP_000554758.1 NZ_CP024886:4621161-4621454 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT89120 NZ_CP024886:4621161-4621454 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3