Detailed information of TA system TA089113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3583975..3584577
• Replicon: chromosome
• Accession: NZ_CP024886
• Organism: Escherichia coli strain AR_0017

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AM344_RS18695
• Protein ID: WP_000897302.1
• Coordinates: 3583975..3584286 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM344_RS18700
• Protein ID: WP_000356397.1
• Coordinates: 3584287..3584577 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM344_RS18665	3579005..3579790	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AM344_RS18670	3579889..3580488	+	600	WP_001314324.1	glucose-1-phosphatase	-
AM344_RS18675	3580482..3581354	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AM344_RS18680	3581351..3581788	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AM344_RS18685	3581833..3582774	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AM344_RS18690	3582838..3583746	-	909	WP_100273025.1	alpha/beta hydrolase	-
AM344_RS18695	3583975..3584286	+	312	WP_000897302.1	hypothetical protein	Toxin
AM344_RS18700	3584287..3584577	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AM344_RS18710	3584936..3585214	+	279	WP_001296612.1	hypothetical protein	-
AM344_RS18720	3585610..3585828	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
AM344_RS18725	3586052..3586981	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AM344_RS18730	3586978..3587613	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AM344_RS18735	3587610..3588512	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T89113 WP_000897302.1 NZ_CP024886:3583975-3584286 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T89113 NZ_CP024886:3583975-3584286 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT89113 WP_000356397.1 NZ_CP024886:3584287-3584577 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT89113 NZ_CP024886:3584287-3584577 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416