Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2225031..2225758 Replicon chromosome
Accession NZ_CP024886
Organism Escherichia coli strain AR_0017

Toxin (Protein)


Gene name higB Uniprot ID A0A8E0MHE9
Locus tag AM344_RS11920 Protein ID WP_021524447.1
Coordinates 2225447..2225758 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AM344_RS11915 Protein ID WP_000126304.1
Coordinates 2225031..2225450 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM344_RS11895 2221194..2223446 - 2253 WP_021524446.1 carbamoyltransferase HypF -
AM344_RS11900 2223599..2224126 - 528 WP_001078777.1 electron transport protein HydN -
AM344_RS11910 2224511..2224939 + 429 WP_000536064.1 hypothetical protein -
AM344_RS11915 2225031..2225450 - 420 WP_000126304.1 helix-turn-helix domain-containing protein Antitoxin
AM344_RS11920 2225447..2225758 - 312 WP_021524447.1 type II toxin-antitoxin system HigB family toxin Toxin
AM344_RS11925 2225921..2226676 - 756 WP_001314074.1 hypothetical protein -
AM344_RS11930 2226719..2227189 - 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
AM344_RS11935 2227182..2227592 - 411 WP_001291913.1 formate hydrogenlyase assembly protein HycH -
AM344_RS11940 2227589..2228356 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
AM344_RS11945 2228356..2228898 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
AM344_RS11950 2228908..2230617 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12428.24 Da        Isoelectric Point: 9.7074

>T89110 WP_021524447.1 NZ_CP024886:c2225758-2225447 [Escherichia coli]
MHIISKAPFEECARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNKLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T89110 NZ_CP024886:c2225758-2225447 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGCGCGCGCAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAAGCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15474.50 Da        Isoelectric Point: 4.4596

>AT89110 WP_000126304.1 NZ_CP024886:c2225450-2225031 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT89110 NZ_CP024886:c2225450-2225031 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References