Detailed information of TA system TA089025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 28937..29664
• Replicon: plasmid pNH25.1
• Accession: NZ_CP024875
• Organism: Klebsiella pneumoniae strain NH25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: CU111_RS27020
• Protein ID: WP_011251285.1
• Coordinates: 28937..29248 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CU111_RS27025
• Protein ID: WP_011251286.1
• Coordinates: 29245..29664 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU111_RS26990	24129..25109	+	981	WP_012569499.1	IS5-like element ISKpn26 family transposase	-
CU111_RS26995	25654..25833	-	180	Protein_29	IS5/IS1182 family transposase	-
CU111_RS27000	25873..27411	-	1539	WP_017901237.1	IS66 family transposase	-
CU111_RS27005	27460..27807	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
CU111_RS27010	27804..28181	-	378	WP_004118218.1	IS66 family insertion sequence hypothetical protein	-
CU111_RS27015	28274..28732	+	459	Protein_33	DDE-type integrase/transposase/recombinase	-
CU111_RS27020	28937..29248	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
CU111_RS27025	29245..29664	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
CU111_RS27030	29734..30147	+	414	WP_017901337.1	hypothetical protein	-
CU111_RS27035	30193..31161	-	969	WP_074168518.1	IS5-like element IS903B family transposase	-
CU111_RS27040	31265..31531	+	267	WP_017900868.1	hypothetical protein	-
CU111_RS27045	31645..32180	-	536	Protein_39	transposase	-
CU111_RS27050	32705..34072	-	1368	WP_017900870.1	formimidoylglutamate deiminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(D) / catA1 / dfrA5 / qacE	-	1..227692	227692
	-	inside	IScluster/Tn	-	-	24129..32039	7910

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T89025 WP_011251285.1 NZ_CP024875:28937-29248 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T89025 NZ_CP024875:28937-29248 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT89025 WP_011251286.1 NZ_CP024875:29245-29664 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT89025 NZ_CP024875:29245-29664 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1