Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2628991..2629588 Replicon chromosome
Accession NZ_CP024874
Organism Klebsiella pneumoniae strain NH25

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag CU111_RS13065 Protein ID WP_004142563.1
Coordinates 2629271..2629588 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag CU111_RS13060 Protein ID WP_004142561.1
Coordinates 2628991..2629278 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU111_RS13030 2625071..2625319 + 249 WP_020325243.1 DUF1158 domain-containing protein -
CU111_RS13035 2625337..2625678 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
CU111_RS13040 2625709..2626824 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
CU111_RS13045 2627004..2627585 + 582 WP_107326660.1 TetR/AcrR family transcriptional regulator -
CU111_RS13050 2627585..2627953 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
CU111_RS13055 2628073..2628726 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
CU111_RS13060 2628991..2629278 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
CU111_RS13065 2629271..2629588 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CU111_RS13070 2629773..2630816 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
CU111_RS13080 2631486..2632352 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
CU111_RS13085 2632461..2633888 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T89014 WP_004142563.1 NZ_CP024874:c2629588-2629271 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T89014 NZ_CP024874:c2629588-2629271 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT89014 WP_004142561.1 NZ_CP024874:c2629278-2628991 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT89014 NZ_CP024874:c2629278-2628991 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References