Detailed information of TA system TA088985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 24500..25146
• Replicon: plasmid unitig_3_pilon
• Accession: NZ_CP024865
• Organism: Escherichia coli strain AR_0015

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM342_RS28045
• Protein ID: WP_000269912.1
• Coordinates: 24799..25146 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM342_RS28040
• Protein ID: WP_001673379.1
• Coordinates: 24500..24799 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM342_RS28000	19680..19928	+	249	WP_000730008.1	hypothetical protein	-
AM342_RS28700	19991..20140	+	150	WP_000003577.1	hypothetical protein	-
AM342_RS28705	20753..20947	+	195	WP_157836022.1	ash family protein	-
AM342_RS28010	21058..21249	+	192	WP_001705001.1	hypothetical protein	-
AM342_RS28015	21246..22388	+	1143	WP_100190852.1	ORF6N domain-containing protein	-
AM342_RS28020	22399..22587	-	189	WP_004105254.1	hypothetical protein	-
AM342_RS28025	23198..23470	-	273	WP_024171748.1	helix-turn-helix domain-containing protein	-
AM342_RS28030	23534..23914	-	381	WP_000061763.1	hypothetical protein	-
AM342_RS28035	24182..24466	+	285	WP_001673380.1	hypothetical protein	-
AM342_RS28040	24500..24799	-	300	WP_001673379.1	XRE family transcriptional regulator	Antitoxin
AM342_RS28045	24799..25146	-	348	WP_000269912.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM342_RS28050	25391..25654	-	264	WP_000424604.1	hypothetical protein	-
AM342_RS28055	25678..25965	-	288	WP_000356589.1	hypothetical protein	-
AM342_RS28060	26609..27445	-	837	WP_024171747.1	replication initiator protein RepA	-
AM342_RS28065	28071..28616	+	546	WP_100190859.1	transferase	-
AM342_RS28070	28580..29197	-	618	WP_001523080.1	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48511	48511

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13545.51 Da
• Isoelectric Point: 8.8337

>T88985 WP_000269912.1 NZ_CP024865:c25146-24799 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T88985 NZ_CP024865:c25146-24799 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCCATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAACGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11069.90 Da
• Isoelectric Point: 5.8899

>AT88985 WP_001673379.1 NZ_CP024865:c24799-24500 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFHV

Nucleotide

• Length: 300 bp

>AT88985 NZ_CP024865:c24799-24500 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCTGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCTACAGGAAAGCGAGTAGCGTTCCATGTCTAA