Detailed information of TA system TA088980

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 130430..130669
• Replicon: plasmid unitig_1_pilon
• Accession: NZ_CP024863
• Organism: Escherichia coli strain AR_0015

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: AM342_RS27235
• Protein ID: WP_023144756.1
• Coordinates: 130430..130564 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	130609..130669 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM342_RS27205	126131..126988	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
AM342_RS27210	126981..127055	-	75	WP_031943482.1	RepA leader peptide Tap	-
AM342_RS28650	127052..127186	-	135	Protein_152	protein CopA/IncA	-
AM342_RS27220	127417..128958	+	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
AM342_RS27225	128973..129719	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
AM342_RS27230	129879..130133	-	255	WP_000083850.1	replication regulatory protein RepA	-
AM342_RS27235	130430..130564	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	130609..130669	+	61	NuclAT_2	-	Antitoxin
-	130609..130669	+	61	NuclAT_2	-	Antitoxin
-	130609..130669	+	61	NuclAT_2	-	Antitoxin
-	130609..130669	+	61	NuclAT_2	-	Antitoxin
AM342_RS28655	130636..130922	-	287	Protein_157	DUF2726 domain-containing protein	-
AM342_RS27250	131435..131647	-	213	WP_013023861.1	hypothetical protein	-
AM342_RS27255	131778..132338	-	561	WP_000139341.1	fertility inhibition protein FinO	-
AM342_RS27260	132393..133139	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(B) / dfrA17 / aadA5 / qacE / sul1 / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B / mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId	-	1..139059	139059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T88980 WP_023144756.1 NZ_CP024863:c130564-130430 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T88980 NZ_CP024863:c130564-130430 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT88980 NZ_CP024863:130609-130669 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin