Detailed information of TA system TA088973

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 12497..12737
• Replicon: plasmid unitig_1_pilon
• Accession: NZ_CP024863
• Organism: Escherichia coli strain AR_0015

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM342_RS26415
• Protein ID: WP_001312861.1
• Coordinates: 12497..12655 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	12699..12737 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM342_RS26370	7609..7836	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
AM342_RS26375	7924..8601	-	678	WP_001348626.1	PAS domain-containing protein	-
AM342_RS26380	8735..9118	-	384	WP_001151566.1	relaxosome protein TraM	-
AM342_RS26385	9449..10051	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
AM342_RS26390	10348..11169	-	822	WP_001234469.1	DUF945 domain-containing protein	-
AM342_RS26395	11288..11575	-	288	WP_000107535.1	hypothetical protein	-
AM342_RS28645	11600..11806	-	207	WP_024190427.1	hypothetical protein	-
AM342_RS26415	12497..12655	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	12699..12737	-	39	NuclAT_1	-	Antitoxin
-	12699..12737	-	39	NuclAT_1	-	Antitoxin
-	12699..12737	-	39	NuclAT_1	-	Antitoxin
-	12699..12737	-	39	NuclAT_1	-	Antitoxin
-	14177..14279	-	103	NuclAT_0	-	-
-	14177..14279	-	103	NuclAT_0	-	-
-	14177..14279	-	103	NuclAT_0	-	-
-	14177..14279	-	103	NuclAT_0	-	-
AM342_RS26425	14291..15010	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
AM342_RS26430	15007..15441	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
AM342_RS26435	15496..17454	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(B) / dfrA17 / aadA5 / qacE / sul1 / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B / mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId	-	1..139059	139059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T88973 WP_001312861.1 NZ_CP024863:c12655-12497 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T88973 NZ_CP024863:c12655-12497 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGAGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 39 bp

>AT88973 NZ_CP024863:c12737-12699 [Escherichia coli]
CATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin