Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3594995..3595689 Replicon chromosome
Accession NZ_CP024862
Organism Escherichia coli strain AR_0015

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag AM342_RS18360 Protein ID WP_001521903.1
Coordinates 3595291..3595689 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag AM342_RS18355 Protein ID WP_000554758.1
Coordinates 3594995..3595288 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM342_RS18330 3590110..3590859 + 750 WP_016233951.1 C40 family peptidase -
AM342_RS18335 3590916..3591269 - 354 WP_024166466.1 type II toxin-antitoxin system HicB family antitoxin -
AM342_RS18340 3591348..3593060 - 1713 Protein_3332 flagellar biosynthesis protein FlhA -
AM342_RS18345 3593032..3593817 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
AM342_RS18350 3593888..3594943 + 1056 WP_001226177.1 DNA polymerase IV -
AM342_RS18355 3594995..3595288 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
AM342_RS18360 3595291..3595689 + 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
AM342_RS18365 3595699..3596151 + 453 WP_023144376.1 GNAT family N-acetyltransferase -
AM342_RS18370 3596396..3596602 + 207 Protein_3338 RtcB family protein -
AM342_RS18375 3596598..3596950 + 353 Protein_3339 peptide chain release factor H -
AM342_RS18380 3597007..3598464 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
AM342_RS18385 3598725..3599183 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
AM342_RS18390 3599275..3600519 + 1245 WP_000189541.1 esterase FrsA -
- 3599779..3599859 + 81 NuclAT_11 - -
- 3599779..3599859 + 81 NuclAT_11 - -
- 3599779..3599859 + 81 NuclAT_11 - -
- 3599779..3599859 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T88960 WP_001521903.1 NZ_CP024862:3595291-3595689 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T88960 NZ_CP024862:3595291-3595689 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT88960 WP_000554758.1 NZ_CP024862:3594995-3595288 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT88960 NZ_CP024862:3594995-3595288 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References