Detailed information of TA system TA088953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2636236..2636838
• Replicon: chromosome
• Accession: NZ_CP024862
• Organism: Escherichia coli strain AR_0015

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AM342_RS13420
• Protein ID: WP_000897305.1
• Coordinates: 2636236..2636547 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM342_RS13425
• Protein ID: WP_000356395.1
• Coordinates: 2636548..2636838 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM342_RS13395	2631277..2631714	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AM342_RS13400	2631759..2632700	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AM342_RS13405	2633116..2634930	+	1815	WP_020233236.1	hypothetical protein	-
AM342_RS13415	2635099..2636007	-	909	WP_001306651.1	alpha/beta hydrolase	-
AM342_RS13420	2636236..2636547	+	312	WP_000897305.1	hypothetical protein	Toxin
AM342_RS13425	2636548..2636838	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
AM342_RS13435	2637197..2637475	+	279	WP_001306650.1	hypothetical protein	-
AM342_RS13445	2637894..2638112	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
AM342_RS13450	2638331..2638573	+	243	WP_021523315.1	hypothetical protein	-
AM342_RS13455	2638755..2639684	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AM342_RS13460	2639681..2640316	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AM342_RS13465	2640313..2641215	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T88953 WP_000897305.1 NZ_CP024862:2636236-2636547 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T88953 NZ_CP024862:2636236-2636547 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT88953 WP_000356395.1 NZ_CP024862:2636548-2636838 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT88953 NZ_CP024862:2636548-2636838 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7