Detailed information of TA system TA088947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1327196..1327923
• Replicon: chromosome
• Accession: NZ_CP024862
• Organism: Escherichia coli strain AR_0015

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: AM342_RS06740
• Protein ID: WP_001521139.1
• Coordinates: 1327612..1327923 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM342_RS06735
• Protein ID: WP_000126294.1
• Coordinates: 1327196..1327615 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM342_RS06715	1322281..1322808	-	528	WP_001078777.1	electron transport protein HydN	-
AM342_RS06720	1322957..1323967	-	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
AM342_RS06725	1324227..1325684	+	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
AM342_RS06730	1325693..1327117	+	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
AM342_RS06735	1327196..1327615	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
AM342_RS06740	1327612..1327923	-	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
AM342_RS06745	1328090..1328560	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
AM342_RS06750	1328553..1328963	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
AM342_RS06755	1328960..1329727	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
AM342_RS06760	1329727..1330269	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
AM342_RS06765	1330279..1331988	-	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T88947 WP_001521139.1 NZ_CP024862:c1327923-1327612 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T88947 NZ_CP024862:c1327923-1327612 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT88947 WP_000126294.1 NZ_CP024862:c1327615-1327196 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT88947 NZ_CP024862:c1327615-1327196 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9