Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 560635..561137 Replicon chromosome
Accession NZ_CP024862
Organism Escherichia coli strain AR_0015

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM342_RS03045 Protein ID WP_000767829.1
Coordinates 560635..560889 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM342_RS03050 Protein ID WP_001259255.1
Coordinates 560886..561137 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM342_RS03015 555650..555877 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
AM342_RS03020 555891..556949 - 1059 WP_000492324.1 transport protein YeeE -
AM342_RS03025 557128..558486 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM342_RS03030 558476..558538 - 63 WP_010723108.1 membrane protein YoeI -
AM342_RS03035 558753..559682 - 930 WP_023154044.1 LysR family transcriptional regulator -
AM342_RS03040 559728..560552 - 825 WP_000754758.1 SDR family oxidoreductase -
AM342_RS03045 560635..560889 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM342_RS03050 560886..561137 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM342_RS03055 561420..561470 + 51 WP_001364200.1 his operon leader peptide -
AM342_RS03065 561616..562515 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
AM342_RS03070 562521..563825 + 1305 WP_021523144.1 histidinol dehydrogenase -
AM342_RS03075 563822..564892 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
AM342_RS03080 564892..565959 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T88946 WP_000767829.1 NZ_CP024862:c560889-560635 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T88946 NZ_CP024862:c560889-560635 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT88946 WP_001259255.1 NZ_CP024862:c561137-560886 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT88946 NZ_CP024862:c561137-560886 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References