Detailed information of TA system TA088925

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 20477..20731
• Replicon: plasmid unitig_1_pilon
• Accession: NZ_CP024860
• Organism: Escherichia coli strain AR_0014

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: AM341_RS24845
• Protein ID: WP_015387399.1
• Coordinates: 20525..20731 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	20477..20538 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM341_RS24795	16129..16341	+	213	WP_005012601.1	hypothetical protein	-
AM341_RS26655	16642..16731	-	90	Protein_14	IS1 family transposase	-
AM341_RS24810	16786..17463	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
AM341_RS24815	17463..17810	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
AM341_RS24820	17830..19401	+	1572	WP_023149734.1	IS66 family transposase	-
AM341_RS24825	19439..20055	-	617	Protein_18	IS1 family transposase	-
AM341_RS24835	20156..20338	+	183	WP_000968309.1	hypothetical protein	-
-	20477..20538	-	62	NuclAT_2	-	Antitoxin
-	20477..20538	-	62	NuclAT_2	-	Antitoxin
-	20477..20538	-	62	NuclAT_2	-	Antitoxin
-	20477..20538	-	62	NuclAT_2	-	Antitoxin
AM341_RS24845	20525..20731	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
AM341_RS24850	21015..21272	+	258	WP_000083833.1	replication regulatory protein RepA	-
AM341_RS24860	21508..21582	+	75	WP_032336874.1	RepA leader peptide Tap	-
AM341_RS24865	21575..22021	+	447	Protein_23	incFII family plasmid replication initiator RepA	-
AM341_RS24870	21967..22449	-	483	Protein_24	VENN motif pre-toxin domain-containing protein	-
AM341_RS24875	23342..24562	+	1221	WP_000410951.1	arginine deiminase	-
AM341_RS24880	24573..25484	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD	senB / iucA / iucB / iucC / iucD / iutA	1..172588	172588

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T88925 WP_015387399.1 NZ_CP024860:20525-20731 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T88925 NZ_CP024860:20525-20731 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT88925 NZ_CP024860:c20538-20477 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin