Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4483290..4483792 Replicon chromosome
Accession NZ_CP024859
Organism Escherichia coli strain AR_0014

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM341_RS22830 Protein ID WP_000767829.1
Coordinates 4483538..4483792 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM341_RS22825 Protein ID WP_001259255.1
Coordinates 4483290..4483541 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM341_RS22795 4478468..4479535 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AM341_RS22800 4479535..4480605 - 1071 WP_000108939.1 histidinol-phosphate transaminase -
AM341_RS22805 4480602..4481906 - 1305 WP_001614652.1 histidinol dehydrogenase -
AM341_RS22810 4481912..4482811 - 900 WP_000131777.1 ATP phosphoribosyltransferase -
AM341_RS22820 4482957..4483007 - 51 WP_001364200.1 his operon leader peptide -
AM341_RS22825 4483290..4483541 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM341_RS22830 4483538..4483792 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM341_RS22835 4483875..4484699 + 825 WP_000754749.1 SDR family oxidoreductase -
AM341_RS22840 4484745..4485674 + 930 WP_000803351.1 LysR family transcriptional regulator -
AM341_RS22845 4485889..4485951 + 63 WP_010723108.1 membrane protein YoeI -
AM341_RS22850 4485941..4487299 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM341_RS22855 4487478..4488536 + 1059 WP_000492321.1 transport protein YeeE -
AM341_RS22860 4488550..4488777 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T88915 WP_000767829.1 NZ_CP024859:4483538-4483792 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T88915 NZ_CP024859:4483538-4483792 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT88915 WP_001259255.1 NZ_CP024859:4483290-4483541 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT88915 NZ_CP024859:4483290-4483541 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References