Detailed information of TA system TA088904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1538654..1539348
• Replicon: chromosome
• Accession: NZ_CP024859
• Organism: Escherichia coli strain AR_0014

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: AM341_RS08000
• Protein ID: WP_001263489.1
• Coordinates: 1538654..1539052 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AM341_RS08005
• Protein ID: WP_000554758.1
• Coordinates: 1539055..1539348 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	1534242..1534322	-	81	NuclAT_11	-	-
-	1534242..1534322	-	81	NuclAT_11	-	-
-	1534242..1534322	-	81	NuclAT_11	-	-
-	1534242..1534322	-	81	NuclAT_11	-	-
AM341_RS07970	1534918..1535376	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
AM341_RS07975	1535637..1537094	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
AM341_RS07980	1537151..1537672	-	522	Protein_1444	peptide chain release factor H	-
AM341_RS07985	1537668..1537874	-	207	Protein_1445	RtcB family protein	-
AM341_RS07995	1538192..1538644	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
AM341_RS08000	1538654..1539052	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AM341_RS08005	1539055..1539348	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AM341_RS08010	1539400..1540455	-	1056	WP_001226164.1	DNA polymerase IV	-
AM341_RS08015	1540526..1541311	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
AM341_RS08020	1541283..1542995	+	1713	Protein_1451	flagellar biosynthesis protein FlhA	-
AM341_RS08025	1543219..1543716	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	1538654..1553934	15280

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T88904 WP_001263489.1 NZ_CP024859:c1539052-1538654 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T88904 NZ_CP024859:c1539052-1538654 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT88904 WP_000554758.1 NZ_CP024859:c1539348-1539055 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT88904 NZ_CP024859:c1539348-1539055 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3