Detailed information of TA system TA088896

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 167946..168200
• Replicon: plasmid tig00001011_pilon
• Accession: NZ_CP024856
• Organism: Escherichia coli strain AR_0011

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: AM338_RS25965
• Protein ID: WP_015387399.1
• Coordinates: 167946..168152 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	168139..168200 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM338_RS25930	163193..164104	-	912	WP_000440183.1	carbamate kinase	-
AM338_RS25935	164115..165335	-	1221	WP_000410951.1	arginine deiminase	-
AM338_RS25940	166228..166710	+	483	Protein_191	VENN motif pre-toxin domain-containing protein	-
AM338_RS25945	166656..167102	-	447	Protein_192	incFII family plasmid replication initiator RepA	-
AM338_RS25950	167095..167169	-	75	WP_032336874.1	RepA leader peptide Tap	-
AM338_RS25960	167405..167662	-	258	WP_000083833.1	replication regulatory protein RepA	-
AM338_RS25965	167946..168152	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	168139..168200	+	62	NuclAT_2	-	Antitoxin
-	168139..168200	+	62	NuclAT_2	-	Antitoxin
-	168139..168200	+	62	NuclAT_2	-	Antitoxin
-	168139..168200	+	62	NuclAT_2	-	Antitoxin
AM338_RS25975	168339..168521	-	183	WP_000968309.1	hypothetical protein	-
AM338_RS25985	168622..169238	+	617	Protein_197	IS1 family transposase	-
AM338_RS25990	169276..170847	-	1572	WP_023149734.1	IS66 family transposase	-
AM338_RS25995	170867..171214	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
AM338_RS26000	171214..171891	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
AM338_RS27410	171946..172035	+	90	Protein_201	IS1 family transposase	-
AM338_RS26015	172336..172548	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / tet(A)	iutA / iucD / iucC / iucB / iucA / senB	1..181436	181436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T88896 WP_015387399.1 NZ_CP024856:c168152-167946 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T88896 NZ_CP024856:c168152-167946 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT88896 NZ_CP024856:168139-168200 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin