Detailed information of TA system TA088890

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26577..26810
• Replicon: plasmid tig00001011_pilon
• Accession: NZ_CP024856
• Organism: Escherichia coli strain AR_0011

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM338_RS25030
• Protein ID: WP_001312861.1
• Coordinates: 26577..26735 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	26779..26810 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM338_RS24995	21951..22640	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
AM338_RS25000	22827..23210	-	384	WP_001151524.1	relaxosome protein TraM	-
AM338_RS25005	23531..24133	+	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
AM338_RS25010	24430..25251	-	822	WP_001234426.1	DUF945 domain-containing protein	-
AM338_RS25015	25369..25656	-	288	WP_000107535.1	hypothetical protein	-
AM338_RS25030	26577..26735	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	26779..26810	-	32	NuclAT_1	-	Antitoxin
-	26779..26810	-	32	NuclAT_1	-	Antitoxin
-	26779..26810	-	32	NuclAT_1	-	Antitoxin
-	26779..26810	-	32	NuclAT_1	-	Antitoxin
-	28250..28352	-	103	NuclAT_0	-	-
-	28250..28352	-	103	NuclAT_0	-	-
-	28250..28352	-	103	NuclAT_0	-	-
-	28250..28352	-	103	NuclAT_0	-	-
AM338_RS25040	28364..29083	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
AM338_RS25045	29080..29514	-	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
AM338_RS25050	29569..29766	-	198	Protein_38	hypothetical protein	-
AM338_RS25055	29794..30027	-	234	WP_000005990.1	DUF905 family protein	-
AM338_RS25060	30095..30634	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
AM338_RS27400	30660..30866	-	207	WP_000275856.1	hypothetical protein	-
AM338_RS25075	31276..31494	-	219	WP_001496291.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / tet(A)	iutA / iucD / iucC / iucB / iucA / senB	1..181436	181436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T88890 WP_001312861.1 NZ_CP024856:c26735-26577 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T88890 NZ_CP024856:c26735-26577 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT88890 NZ_CP024856:c26810-26779 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin