Detailed information of TA system TA088887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4527863..4528557
• Replicon: chromosome
• Accession: NZ_CP024855
• Organism: Escherichia coli strain AR_0011

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: AM338_RS23115
• Protein ID: WP_001263489.1
• Coordinates: 4528159..4528557 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AM338_RS23110
• Protein ID: WP_000554758.1
• Coordinates: 4527863..4528156 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM338_RS23090	4523495..4523992	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
AM338_RS23095	4524216..4525928	-	1713	Protein_4218	flagellar biosynthesis protein FlhA	-
AM338_RS23100	4525900..4526685	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
AM338_RS23105	4526756..4527811	+	1056	WP_001226164.1	DNA polymerase IV	-
AM338_RS23110	4527863..4528156	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AM338_RS23115	4528159..4528557	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AM338_RS23120	4528567..4529019	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
AM338_RS23125	4529337..4529543	+	207	Protein_4224	RtcB family protein	-
AM338_RS23130	4529539..4530060	+	522	Protein_4225	peptide chain release factor H	-
AM338_RS23135	4530117..4531574	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
AM338_RS23140	4531835..4532293	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	4532889..4532969	+	81	NuclAT_11	-	-
-	4532889..4532969	+	81	NuclAT_11	-	-
-	4532889..4532969	+	81	NuclAT_11	-	-
-	4532889..4532969	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	4514100..4546845	32745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T88887 WP_001263489.1 NZ_CP024855:4528159-4528557 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T88887 NZ_CP024855:4528159-4528557 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT88887 WP_000554758.1 NZ_CP024855:4527863-4528156 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT88887 NZ_CP024855:4527863-4528156 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3