Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1584500..1585002 Replicon chromosome
Accession NZ_CP024855
Organism Escherichia coli strain AR_0011

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM338_RS08285 Protein ID WP_000767829.1
Coordinates 1584500..1584754 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM338_RS08290 Protein ID WP_001259255.1
Coordinates 1584751..1585002 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM338_RS08255 1579515..1579742 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
AM338_RS08260 1579756..1580814 - 1059 WP_000492321.1 transport protein YeeE -
AM338_RS08265 1580993..1582351 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM338_RS08270 1582341..1582403 - 63 WP_010723108.1 membrane protein YoeI -
AM338_RS08275 1582618..1583547 - 930 WP_000803351.1 LysR family transcriptional regulator -
AM338_RS08280 1583593..1584417 - 825 WP_000754749.1 SDR family oxidoreductase -
AM338_RS08285 1584500..1584754 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM338_RS08290 1584751..1585002 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM338_RS08295 1585285..1585335 + 51 WP_001364200.1 his operon leader peptide -
AM338_RS08305 1585481..1586380 + 900 WP_000131777.1 ATP phosphoribosyltransferase -
AM338_RS08310 1586386..1587690 + 1305 WP_001614652.1 histidinol dehydrogenase -
AM338_RS08315 1587687..1588757 + 1071 WP_000108939.1 histidinol-phosphate transaminase -
AM338_RS08320 1588757..1589824 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T88876 WP_000767829.1 NZ_CP024855:c1584754-1584500 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T88876 NZ_CP024855:c1584754-1584500 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT88876 WP_001259255.1 NZ_CP024855:c1585002-1584751 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT88876 NZ_CP024855:c1585002-1584751 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References