Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4042569..4043071 Replicon chromosome
Accession NZ_CP024851
Organism Escherichia coli strain AR_0006

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM333_RS21035 Protein ID WP_000767829.1
Coordinates 4042569..4042823 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM333_RS21040 Protein ID WP_001259255.1
Coordinates 4042820..4043071 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM333_RS21005 4037584..4037811 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
AM333_RS21010 4037825..4038883 - 1059 WP_000492324.1 transport protein YeeE -
AM333_RS21015 4039062..4040420 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM333_RS21020 4040410..4040472 - 63 WP_010723108.1 membrane protein YoeI -
AM333_RS21025 4040687..4041616 - 930 WP_023154044.1 LysR family transcriptional regulator -
AM333_RS21030 4041662..4042486 - 825 WP_000754758.1 SDR family oxidoreductase -
AM333_RS21035 4042569..4042823 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM333_RS21040 4042820..4043071 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM333_RS21045 4043354..4043404 + 51 WP_001364200.1 his operon leader peptide -
AM333_RS21055 4043550..4044449 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
AM333_RS21060 4044455..4045759 + 1305 WP_021523144.1 histidinol dehydrogenase -
AM333_RS21065 4045756..4046826 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
AM333_RS21070 4046826..4047893 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T88849 WP_000767829.1 NZ_CP024851:c4042823-4042569 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T88849 NZ_CP024851:c4042823-4042569 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT88849 WP_001259255.1 NZ_CP024851:c4043071-4042820 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT88849 NZ_CP024851:c4043071-4042820 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References