Detailed information of TA system TA088827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2010573..2011267
• Replicon: chromosome
• Accession: NZ_CP024851
• Organism: Escherichia coli strain AR_0006

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: AM333_RS10355
• Protein ID: WP_001521903.1
• Coordinates: 2010869..2011267 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AM333_RS10350
• Protein ID: WP_000554758.1
• Coordinates: 2010573..2010866 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM333_RS10325	2005688..2006437	+	750	WP_016233951.1	C40 family peptidase	-
AM333_RS10330	2006494..2006847	-	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
AM333_RS10335	2006926..2008638	-	1713	Protein_1847	flagellar biosynthesis protein FlhA	-
AM333_RS10340	2008610..2009395	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
AM333_RS10345	2009466..2010521	+	1056	WP_001226177.1	DNA polymerase IV	-
AM333_RS10350	2010573..2010866	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AM333_RS10355	2010869..2011267	+	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AM333_RS10360	2011277..2011729	+	453	WP_023144376.1	GNAT family N-acetyltransferase	-
AM333_RS10365	2011974..2012180	+	207	Protein_1853	RtcB family protein	-
AM333_RS10370	2012176..2012528	+	353	Protein_1854	peptide chain release factor H	-
AM333_RS10375	2012585..2014042	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
AM333_RS10380	2014303..2014761	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
AM333_RS10385	2014853..2016097	+	1245	WP_000189541.1	esterase FrsA	-
-	2015357..2015437	+	81	NuclAT_11	-	-
-	2015357..2015437	+	81	NuclAT_11	-	-
-	2015357..2015437	+	81	NuclAT_11	-	-
-	2015357..2015437	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T88827 WP_001521903.1 NZ_CP024851:2010869-2011267 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T88827 NZ_CP024851:2010869-2011267 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT88827 WP_000554758.1 NZ_CP024851:2010573-2010866 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT88827 NZ_CP024851:2010573-2010866 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3