Detailed information of TA system TA088821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1122902..1123504
• Replicon: chromosome
• Accession: NZ_CP024851
• Organism: Escherichia coli strain AR_0006

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AM333_RS05845
• Protein ID: WP_000897305.1
• Coordinates: 1122902..1123213 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM333_RS05850
• Protein ID: WP_000356395.1
• Coordinates: 1123214..1123504 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM333_RS05820	1117943..1118380	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AM333_RS05825	1118425..1119366	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AM333_RS05830	1119782..1121596	+	1815	WP_020233236.1	hypothetical protein	-
AM333_RS05840	1121765..1122673	-	909	WP_001306651.1	alpha/beta hydrolase	-
AM333_RS05845	1122902..1123213	+	312	WP_000897305.1	hypothetical protein	Toxin
AM333_RS05850	1123214..1123504	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
AM333_RS05855	1123589..1123801	+	213	WP_000197769.1	hypothetical protein	-
AM333_RS05860	1123863..1124141	+	279	WP_001306650.1	hypothetical protein	-
AM333_RS05870	1124560..1124778	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
AM333_RS05875	1124997..1125239	+	243	WP_021523315.1	hypothetical protein	-
AM333_RS05880	1125421..1126350	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AM333_RS05885	1126347..1126982	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AM333_RS05890	1126979..1127881	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T88821 WP_000897305.1 NZ_CP024851:1122902-1123213 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T88821 NZ_CP024851:1122902-1123213 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT88821 WP_000356395.1 NZ_CP024851:1123214-1123504 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT88821 NZ_CP024851:1123214-1123504 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7