Detailed information of TA system TA088806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3050867..3051527
• Replicon: chromosome
• Accession: NZ_CP024842
• Organism: Pectobacterium versatile strain 3-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A221T809
• Locus tag: OA04_RS13975
• Protein ID: WP_044203475.1
• Coordinates: 3051174..3051527 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OA04_RS13970
• Protein ID: WP_010298722.1
• Coordinates: 3050867..3051169 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OA04_RS13940	3047071..3047664	+	594	WP_103862008.1	flavodoxin	-
OA04_RS13945	3047732..3048367	-	636	WP_107333072.1	NAD(P)H-binding protein	-
OA04_RS13950	3048487..3048912	-	426	WP_039540880.1	DoxX family protein	-
OA04_RS13955	3049002..3049394	+	393	WP_103971234.1	helix-turn-helix transcriptional regulator	-
OA04_RS13960	3049422..3049772	-	351	WP_103971201.1	helix-turn-helix domain-containing protein	-
OA04_RS13965	3049825..3050829	+	1005	WP_107333073.1	threonine/serine dehydratase	-
OA04_RS13970	3050867..3051169	-	303	WP_010298722.1	XRE family transcriptional regulator	Antitoxin
OA04_RS13975	3051174..3051527	-	354	WP_044203475.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OA04_RS13980	3051754..3052194	-	441	WP_044203474.1	helix-turn-helix transcriptional regulator	-
OA04_RS13985	3052297..3052908	+	612	WP_039492687.1	NAD(P)-dependent oxidoreductase	-
OA04_RS13990	3053065..3053904	+	840	WP_044203470.1	glutathione-dependent disulfide-bond oxidoreductase	-
OA04_RS13995	3053950..3055206	-	1257	WP_107333074.1	multidrug effflux MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13609.59 Da
• Isoelectric Point: 9.8031

>T88806 WP_044203475.1 NZ_CP024842:c3051527-3051174 [Pectobacterium versatile]
VWRIETTDVFDSWFSALNDRDRACVLAALIVLREKGPMLSRPYADTVKGSRYSNMKELRIQSRGTPIRAFFAFDPHRTGI
LLCAGNKSGNEKRFYDEMLPFAERELTNYLKKSEKRE

Nucleotide

• Length: 354 bp

>T88806 NZ_CP024842:c3051527-3051174 [Pectobacterium versatile]
GTGTGGAGAATTGAAACGACGGACGTGTTTGATAGCTGGTTCAGCGCCTTAAACGATAGGGATCGAGCTTGCGTGCTTGC
AGCACTTATCGTGTTGCGGGAAAAAGGTCCAATGTTATCTAGACCTTATGCCGATACGGTTAAAGGTTCTCGTTATAGCA
ACATGAAAGAGCTGCGTATTCAGAGCCGAGGAACGCCGATACGGGCATTTTTTGCCTTCGACCCACATCGTACAGGGATC
CTGCTCTGCGCAGGCAATAAGTCCGGCAATGAAAAGCGCTTTTACGATGAGATGCTCCCGTTTGCTGAGCGGGAGTTAAC
AAATTATCTGAAAAAATCAGAGAAGAGGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11282.16 Da
• Isoelectric Point: 5.6573

>AT88806 WP_010298722.1 NZ_CP024842:c3051169-3050867 [Pectobacterium versatile]
MGRTLEQLLADEKPEVVVDAHAIATEMLLNIHLAELRERVQKTQVEMAQVLGLKQPTVAVMEKPGRDLKLSTLKRYVEAT
GSKLRLDVELPDGSHYEFML

Nucleotide

• Length: 303 bp

>AT88806 NZ_CP024842:c3051169-3050867 [Pectobacterium versatile]
ATGGGCAGAACGCTGGAACAGCTTCTTGCGGATGAAAAACCAGAGGTTGTGGTCGATGCTCACGCTATAGCGACAGAGAT
GCTGCTCAATATTCATCTCGCTGAACTGCGTGAGAGGGTGCAAAAAACGCAAGTAGAAATGGCACAGGTGCTTGGGTTAA
AACAGCCGACCGTGGCGGTAATGGAGAAACCCGGCCGAGATCTGAAACTTTCCACGCTTAAACGCTATGTCGAAGCGACC
GGCAGTAAGCTACGTCTTGATGTTGAACTGCCGGACGGCTCGCATTACGAATTTATGCTGTAG

Structures

Toxin

• AlphaFold DB: A0A221T809