Detailed information of TA system TA088797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 453488..454212
• Replicon: chromosome
• Accession: NZ_CP024842
• Organism: Pectobacterium versatile strain 3-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A093VC08
• Locus tag: OA04_RS02060
• Protein ID: WP_039482109.1
• Coordinates: 453488..453799 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OA04_RS02065
• Protein ID: WP_103861592.1
• Coordinates: 453796..454212 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OA04_RS02040	448947..449681	+	735	WP_107332211.1	type I-E CRISPR-associated protein Cas5/CasD	-
OA04_RS02045	449681..450328	+	648	WP_107332212.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
OA04_RS02050	450356..451273	+	918	WP_107332213.1	type I-E CRISPR-associated endonuclease Cas1	-
OA04_RS02055	451274..451567	+	294	WP_005973730.1	type I-E CRISPR-associated endoribonuclease Cas2	-
OA04_RS02060	453488..453799	+	312	WP_039482109.1	type II toxin-antitoxin system HigB family toxin	Toxin
OA04_RS02065	453796..454212	+	417	WP_103861592.1	helix-turn-helix domain-containing protein	Antitoxin
OA04_RS02070	454419..454922	-	504	WP_103861591.1	hypothetical protein	-
OA04_RS02075	455268..455504	+	237	WP_103971373.1	type I-E CRISPR-associated endoribonuclease Cas2	-
OA04_RS02080	458671..459129	+	459	WP_039534732.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.29 Da
• Isoelectric Point: 9.8530

>T88797 WP_039482109.1 NZ_CP024842:453488-453799 [Pectobacterium versatile]
MHVISRAPFDTATTQFPNQAAALDDLYRVIKREMYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKI
FIKHITSHAEYDRLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T88797 NZ_CP024842:453488-453799 [Pectobacterium versatile]
ATGCATGTTATTTCGCGCGCACCTTTCGACACTGCAACCACTCAGTTTCCGAATCAGGCGGCAGCACTTGATGACCTATA
TCGGGTTATCAAACGCGAAATGTATGCAACGCCGGACGATATGAAAAAACGCTTCCCTAGTCTAGATAGGATGAAATATC
GGGAAAAATGGTGGGTTATTGATATTGGTGGCGGGCATCTTCGAGTGATGTTTTTTGCTGATTTCGAGCGGGGGAAAATC
TTCATCAAGCACATCACATCCCATGCAGAGTACGACAGGCTGACAGAGTATTACCGGAGGAATAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15405.70 Da
• Isoelectric Point: 4.8037

>AT88797 WP_103861592.1 NZ_CP024842:453796-454212 [Pectobacterium versatile]
MMYADAIKAANNLTSIVPFLGGSTSRKDYEDALKLTEYLIESEPDSPLIDMLTAKIDKYEDDAPEFAAFNERVKALPAGI
ALLRVLMDQHQLTQSDFEAEIGKKSLVSRILKGERTLTLDHMRALAKRFNIPVSAFVD

Nucleotide

• Length: 417 bp

>AT88797 NZ_CP024842:453796-454212 [Pectobacterium versatile]
ATGATGTACGCCGACGCCATCAAGGCAGCTAACAACCTGACGAGTATCGTACCATTCCTCGGAGGCAGCACCTCTCGTAA
GGATTATGAGGATGCACTGAAACTCACTGAATACCTGATCGAGAGTGAACCTGACAGTCCGCTAATCGATATGCTGACTG
CCAAAATTGACAAATACGAAGATGATGCACCGGAGTTTGCAGCCTTCAACGAACGTGTTAAAGCACTCCCAGCAGGTATC
GCTTTACTGCGCGTCCTTATGGATCAGCATCAGCTAACGCAGAGTGATTTTGAAGCTGAGATTGGTAAAAAGTCTCTGGT
ATCCCGCATTCTAAAAGGTGAGCGCACTCTAACGCTGGATCACATGAGAGCGCTTGCTAAACGATTCAATATTCCCGTGA
GTGCTTTTGTTGATTGA

Structures

Toxin

• AlphaFold DB: A0A093VC08