Detailed information of TA system TA088789

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37637..38063
• Replicon: plasmid pCRKP-1215_2
• Accession: NZ_CP024840
• Organism: Klebsiella pneumoniae strain CRKP-1215

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CU074_RS29415
• Protein ID: WP_001312861.1
• Coordinates: 37637..37795 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	37839..38063 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU074_RS28255	32660..32887	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
CU074_RS28260	33024..33695	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
CU074_RS28265	33889..34272	-	384	WP_001354030.1	relaxosome protein TraM	-
CU074_RS28275	34595..35197	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
CU074_RS28280	35494..36315	-	822	WP_001234445.1	DUF945 domain-containing protein	-
CU074_RS28285	36426..36722	-	297	WP_001272251.1	hypothetical protein	-
CU074_RS30040	37022..37399	+	378	Protein_41	hypothetical protein	-
CU074_RS29415	37637..37795	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	37839..38063	-	225	NuclAT_0	-	Antitoxin
-	37839..38063	-	225	NuclAT_0	-	Antitoxin
-	37839..38063	-	225	NuclAT_0	-	Antitoxin
-	37839..38063	-	225	NuclAT_0	-	Antitoxin
CU074_RS28310	38075..38794	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
CU074_RS28315	38791..39225	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CU074_RS28320	39294..41317	-	2024	Protein_45	ParB/RepB/Spo0J family partition protein	-
CU074_RS28325	41378..41611	-	234	WP_000006003.1	DUF905 family protein	-
CU074_RS28330	41669..42196	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A) / erm(B)	-	1..96185	96185

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T88789 WP_001312861.1 NZ_CP024840:c37795-37637 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T88789 NZ_CP024840:c37795-37637 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT88789 NZ_CP024840:c38063-37839 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin