Detailed information of TA system TA088766

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 16938..17364
• Replicon: plasmid pCRKP-2297_2
• Accession: NZ_CP024836
• Organism: Klebsiella pneumoniae strain CRKP-2297

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CU075_RS29280
• Protein ID: WP_001312861.1
• Coordinates: 16938..17096 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	17140..17364 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU075_RS28090	11961..12188	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
CU075_RS28095	12325..12996	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
CU075_RS28100	13190..13573	-	384	WP_001354030.1	relaxosome protein TraM	-
CU075_RS28110	13896..14498	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
CU075_RS28115	14795..15616	-	822	WP_001234445.1	DUF945 domain-containing protein	-
CU075_RS28120	15727..16023	-	297	WP_001272251.1	hypothetical protein	-
CU075_RS29870	16323..16700	+	378	Protein_24	hypothetical protein	-
CU075_RS29280	16938..17096	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	17140..17364	-	225	NuclAT_0	-	Antitoxin
-	17140..17364	-	225	NuclAT_0	-	Antitoxin
-	17140..17364	-	225	NuclAT_0	-	Antitoxin
-	17140..17364	-	225	NuclAT_0	-	Antitoxin
CU075_RS28145	17376..18095	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
CU075_RS28150	18092..18526	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CU075_RS28155	18595..20618	-	2024	Protein_28	ParB/RepB/Spo0J family partition protein	-
CU075_RS28160	20679..20912	-	234	WP_000006003.1	DUF905 family protein	-
CU075_RS28165	20970..21497	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A) / erm(B)	-	1..96185	96185

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T88766 WP_001312861.1 NZ_CP024836:c17096-16938 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T88766 NZ_CP024836:c17096-16938 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT88766 NZ_CP024836:c17364-17140 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin