Detailed information of TA system TA088739

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 101211..101632
• Replicon: plasmid pCREC-532_1
• Accession: NZ_CP024831
• Organism: Escherichia coli strain CREC-532

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: CU076_RS25620
• Protein ID: WP_001302184.1
• Coordinates: 101474..101632 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	101211..101409 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU076_RS25580	97331..97684	+	354	WP_000005489.1	hypothetical protein	-
CU076_RS25585	98097..98675	-	579	WP_032147503.1	DUF2726 domain-containing protein	-
CU076_RS25595	98708..99401	+	694	Protein_121	IS1 family transposase	-
CU076_RS25600	99417..100040	+	624	WP_000912556.1	potassium channel family protein	-
CU076_RS25605	100125..100376	+	252	Protein_123	iron-containing alcohol dehydrogenase	-
CU076_RS25610	100665..100982	-	318	Protein_124	IS110 family transposase	-
CU076_RS25615	101137..101242	+	106	Protein_125	plasmid SOS inhibition protein A	-
-	101211..101409	+	199	NuclAT_0	-	Antitoxin
-	101211..101409	+	199	NuclAT_0	-	Antitoxin
-	101211..101409	+	199	NuclAT_0	-	Antitoxin
-	101211..101409	+	199	NuclAT_0	-	Antitoxin
CU076_RS25620	101474..101632	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
CU076_RS27885	102300..102506	+	207	WP_000275854.1	hypothetical protein	-
CU076_RS25630	102531..102818	+	288	WP_000107242.1	hypothetical protein	-
CU076_RS25635	102937..103758	+	822	WP_001234465.1	DUF945 domain-containing protein	-
CU076_RS25640	104054..104626	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
CU076_RS25645	104996..105379	+	384	WP_001063020.1	relaxosome protein TraM	-
CU076_RS25650	105570..106217	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
CU076_RS25655	106353..106568	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / erm(B) / tet(B) / aac(3)-IId / blaTEM-1B	-	1..216181	216181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T88739 WP_001302184.1 NZ_CP024831:101474-101632 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T88739 NZ_CP024831:101474-101632 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT88739 NZ_CP024831:101211-101409 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin