Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3885142..3885644 Replicon chromosome
Accession NZ_CP024830
Organism Escherichia coli strain CREC-532

Toxin (Protein)


Gene name yoeB Uniprot ID A0A8E0FJZ4
Locus tag CU076_RS20295 Protein ID WP_000767833.1
Coordinates 3885142..3885396 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CU076_RS20300 Protein ID WP_001259255.1
Coordinates 3885393..3885644 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU076_RS20265 3880157..3880384 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CU076_RS20270 3880398..3881456 - 1059 WP_000492324.1 transport protein YeeE -
CU076_RS20275 3881635..3882993 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CU076_RS20280 3882983..3883045 - 63 WP_010723108.1 membrane protein YoeI -
CU076_RS20285 3883260..3884189 - 930 WP_000803351.1 LysR family transcriptional regulator -
CU076_RS20290 3884235..3885059 - 825 WP_000754758.1 SDR family oxidoreductase -
CU076_RS20295 3885142..3885396 - 255 WP_000767833.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CU076_RS20300 3885393..3885644 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CU076_RS20305 3885927..3885977 + 51 WP_001364200.1 his operon leader peptide -
CU076_RS20315 3886123..3887022 + 900 WP_000131756.1 ATP phosphoribosyltransferase -
CU076_RS20320 3887028..3888326 + 1299 WP_049290931.1 histidinol dehydrogenase -
CU076_RS20325 3888329..3889399 + 1071 WP_000108939.1 histidinol-phosphate transaminase -
CU076_RS20330 3889399..3890466 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10173.54 Da        Isoelectric Point: 8.0353

>T88733 WP_000767833.1 NZ_CP024830:c3885396-3885142 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T88733 NZ_CP024830:c3885396-3885142 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAATCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT88733 WP_001259255.1 NZ_CP024830:c3885644-3885393 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT88733 NZ_CP024830:c3885644-3885393 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References