Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1925867..1926561 Replicon chromosome
Accession NZ_CP024830
Organism Escherichia coli strain CREC-532

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag CU076_RS09820 Protein ID WP_001263489.1
Coordinates 1926163..1926561 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag CU076_RS09815 Protein ID WP_000554758.1
Coordinates 1925867..1926160 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU076_RS09795 1921499..1921996 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
CU076_RS09800 1922220..1923932 - 1713 Protein_1781 flagellar biosynthesis protein FlhA -
CU076_RS09805 1923904..1924689 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
CU076_RS09810 1924760..1925815 + 1056 WP_001226164.1 DNA polymerase IV -
CU076_RS09815 1925867..1926160 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CU076_RS09820 1926163..1926561 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CU076_RS09825 1926571..1927023 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
CU076_RS09830 1927341..1927547 + 207 Protein_1787 RtcB family protein -
CU076_RS09835 1927543..1928064 + 522 Protein_1788 peptide chain release factor H -
CU076_RS09840 1928121..1929578 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
CU076_RS09845 1929839..1930297 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 1930893..1930973 + 81 NuclAT_13 - -
- 1930893..1930973 + 81 NuclAT_13 - -
- 1930893..1930973 + 81 NuclAT_13 - -
- 1930893..1930973 + 81 NuclAT_13 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T88718 WP_001263489.1 NZ_CP024830:1926163-1926561 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T88718 NZ_CP024830:1926163-1926561 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT88718 WP_000554758.1 NZ_CP024830:1925867-1926160 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT88718 NZ_CP024830:1925867-1926160 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References