Detailed information of TA system TA088705

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 111941..112367
• Replicon: plasmid pCREC-544_1
• Accession: NZ_CP024827
• Organism: Escherichia coli strain CREC-544

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CU077_RS25440
• Protein ID: WP_001312861.1
• Coordinates: 111941..112099 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	112143..112367 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU077_RS25400	107316..108005	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
CU077_RS25405	108192..108575	-	384	WP_001151524.1	relaxosome protein TraM	-
CU077_RS25410	108896..109498	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
CU077_RS25415	109795..110616	-	822	WP_001234426.1	DUF945 domain-containing protein	-
CU077_RS25420	110734..111021	-	288	WP_000107535.1	hypothetical protein	-
CU077_RS25440	111941..112099	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	112143..112367	-	225	NuclAT_0	-	Antitoxin
-	112143..112367	-	225	NuclAT_0	-	Antitoxin
-	112143..112367	-	225	NuclAT_0	-	Antitoxin
-	112143..112367	-	225	NuclAT_0	-	Antitoxin
CU077_RS26325	112179..112367	+	189	WP_001299721.1	hypothetical protein	-
CU077_RS25445	112379..113098	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
CU077_RS25450	113095..113529	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
CU077_RS25455	113584..113781	-	198	Protein_134	hypothetical protein	-
CU077_RS25460	113809..114042	-	234	WP_000005987.1	DUF905 family protein	-
CU077_RS25465	114110..114649	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
CU077_RS26470	114675..114881	-	207	WP_000275856.1	hypothetical protein	-
CU077_RS25490	116020..116991	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aph(3')-Ia / catA2 / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-55	iucC / iucB / iucA / iutA / iucD	1..122937	122937

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T88705 WP_001312861.1 NZ_CP024827:c112099-111941 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T88705 NZ_CP024827:c112099-111941 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT88705 NZ_CP024827:c112367-112143 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin