Detailed information of TA system TA088700

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 73613..73867
• Replicon: plasmid pCREC-544_1
• Accession: NZ_CP024827
• Organism: Escherichia coli strain CREC-544

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CU077_RS25205
• Protein ID: WP_001351576.1
• Coordinates: 73613..73819 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	73806..73867 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU077_RS25165	69165..69566	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
CU077_RS26320	69499..69756	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
CU077_RS25170	69849..70502	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
CU077_RS26460	70600..70740	-	141	WP_001333237.1	hypothetical protein	-
CU077_RS25180	71441..72298	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
CU077_RS25185	72291..72773	-	483	WP_001273588.1	hypothetical protein	-
CU077_RS25190	72766..72840	-	75	WP_001442103.1	RepA leader peptide Tap	-
CU077_RS25200	73072..73329	-	258	WP_000083845.1	replication regulatory protein RepA	-
CU077_RS25205	73613..73819	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	73806..73867	+	62	NuclAT_1	-	Antitoxin
-	73806..73867	+	62	NuclAT_1	-	Antitoxin
-	73806..73867	+	62	NuclAT_1	-	Antitoxin
-	73806..73867	+	62	NuclAT_1	-	Antitoxin
CU077_RS26465	74123..74197	-	75	Protein_87	endonuclease	-
CU077_RS25215	74443..74655	-	213	WP_001299730.1	ANR family transcriptional regulator	-
CU077_RS25220	74791..75351	-	561	WP_000139315.1	fertility inhibition protein FinO	-
CU077_RS25225	75454..76314	-	861	WP_000704513.1	alpha/beta hydrolase	-
CU077_RS25230	76373..77119	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aph(3')-Ia / catA2 / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-55	iucC / iucB / iucA / iutA / iucD	1..122937	122937

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T88700 WP_001351576.1 NZ_CP024827:c73819-73613 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T88700 NZ_CP024827:c73819-73613 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT88700 NZ_CP024827:73806-73867 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin