Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2547242..2547804 Replicon chromosome
Accession NZ_CP024826
Organism Escherichia coli strain CREC-544

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag CU077_RS12930 Protein ID WP_000605676.1
Coordinates 2547242..2547520 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag CU077_RS12935 Protein ID WP_000781370.1
Coordinates 2547520..2547804 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU077_RS12900 2542828..2543259 - 432 WP_000152305.1 peroxiredoxin OsmC -
CU077_RS12910 2543604..2543819 + 216 WP_000495766.1 biofilm-dependent modulation protein -
CU077_RS12915 2543921..2544058 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
CU077_RS12920 2544215..2545912 + 1698 WP_000433464.1 malate dehydrogenase -
CU077_RS12925 2546046..2547056 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
CU077_RS12930 2547242..2547520 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CU077_RS12935 2547520..2547804 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
CU077_RS12940 2547855..2548508 - 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
CU077_RS12945 2548501..2549385 - 885 WP_001240582.1 formate dehydrogenase N subunit beta -
CU077_RS12950 2549398..2552445 - 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T88691 WP_000605676.1 NZ_CP024826:2547242-2547520 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T88691 NZ_CP024826:2547242-2547520 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCAGATATCGATATGCCCGGTTACCGATTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTCGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT88691 WP_000781370.1 NZ_CP024826:2547520-2547804 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT88691 NZ_CP024826:2547520-2547804 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References